250 Grlomerella cingulata ami iti< Conldial Foi'ms 



■e> 



producing cultures are cultivated on the same food and under the same 

 conditions, they again become sterile. But, though the lost conidial form 

 can be recovered, so far all attempts to get back the perithecial form from 

 the strain which has lost it have been unsuccessful. What factor or 

 factors influence the development of the perfect stage is not known. 



Since 1914, Glceosporium pipemtum E. and E. has been obtained in 

 cultures on several occasions and cultivated on numerous kinds of 

 media but the perithecial stage has been developed only on two occasions. 

 In April 1915, perithecia were first formed on partly sterilized chilli 

 stems. But unfortunately this strain was allowed to die out. In 

 December of 1918, cultures of G. piperatum were taken from diseased 

 chilli fruits in Burma by Babu P. C. Kar, Fieldman to the Imperial 

 Mycologist, to whom my acknowledgements are due. Cultures on 

 glucose-meat-extract-agar and on corn meal produced both the perithecial 

 and Glceosporium forms at Pusa. 



In the winter of 1916, the Glomerella stage was for the first time ob- 

 served on diseased chilli fruits; a few fruits attacked by Globosporium 

 piperatum were incubated. The concentric rings of shining oily pink 

 acervuli on one of the fruits were replaced in five or six days by an un- 

 dulating, black, rugged crust in which were found the perithecia. In 1917 

 and 1918 perithecia were again found on a few incubated fruits attacked 

 by Glceosporium piperatum. 



The perithecial form found on these incubated fruits was identical 

 with that previously produced in cultures of Glceosporium piperatum on 

 partly sterilized chilli stems and on glucose-meat-extract-agar and that 

 described by Miss Stoneman. 



The perithecia found on the fruits (Fig. 4) are caespitose, membra- 

 naceous, pear-shaped with a short neck, dark brown in colour — light 

 coloured towards the ostiole — situated on, or partly immersed in, a 

 stroma of loosely inter-woven hyphae. They are not hairy, but the neck 

 in some cases is slightly tufted. The perithecia resemble those of Glo- 

 merella piperata described by Miss Stoneman exce])t that they are not 

 hairy as figured by her. 



Cultures taken from diseased fruits bearing perithecia have given 

 the ascogenous stage. On maize-agar the perithecia are either scattered 

 or aggregated together forming big black nodules like those of G. frusti- 

 gena (Clint.) Sacc.^. These on sectioning are found to be somewhat 

 differentiated into cortex and medulla. The cortex is composed of large 



' Kdj^erton, (!. W. The Physiology and Develo])iiu'nt of sonu' .Aiitluaciuiso.s. liol. Clitz., 

 xr.v, p. 387, 1908. 



