Jehangir Fardunji Dastur 253 



are not sufficient reasons to consider the ascogenous stage of Gloeosporium 

 'pi'peratum E. and E. to be different from Glomerella cingulata (Stoneiiian) 

 Spauld. and v. Sch. 



Dcrelopmevf o/ Gkposporium and CoUetotrichuni acervuU in 

 cultures of Glomerella. 



In December 1918, a chilli fruit was found attacked by Gloeosporium 

 piperatutn E. and E.; a week after it was incubated, the shining pink 

 acervuli were replaced by a black, corrugated, rugged, carbonaceous 

 crust. In this crust was found the ascogenous stage. A small bit from 

 this perithecial crust was planted in a tube of glucose-meat-extract-agar. 

 The growth remained sterile. In sub-cultures from this culture (which 

 we shall call A) on sterilized chilli stems and on maize-meal-agar, conidia 

 and perithecia were produced, but setae were invariably absent; but, 

 however, sub-cultures on glucose-meat-extract-agar were sterile. Four 

 months later, i.e. in April of the following year, sub-cultures from the 

 sterile culture A were made on sterilized chilli stems (culture B) and on 

 glucose-meat-extract-agar (culture C). In B the perithecial stage was 

 produced, but not the conidial; while in C acervuli with or without 

 setae were developed but no perithecia. 



Thus all of a sudden the original sterile culture broke up into two 

 different strains, one producing only perithecia on sterilized chilli stems 

 and the other forming acervuli with and without setae on glucose-meat- 

 extract-agar. These strains remained distinct only for a short time. The 

 conidial strain soon lost its setae-producing faculty and developed the 

 perithecia-forming faculty on glucose-meat-extract-agar. Subsequent 

 sub-cultures on glucose-meat-extract-agar from these two strains were 

 identical. 



Sub-cultures from the perithecial strain, culture B, continued to 

 produce perithecia, at first along with the GloBosporium stage, on various 

 media, including glucose-meat-extract-agar. Eventually this strain 

 produced only the perfect stage, without the conidial. 



Sub-cultures from the conidial strain, culture C, on sterilized chilli 

 stems, oat-juice-agar and maize-agar, at first, gave both the perithecial 

 stage and the conidial, either with or without setae, but later only the 

 perfect stage was produced on these media. Sub-cultures on glucose- 

 meat-extract-agar, ± 5 Fuller's scale and neutral, and on bouillon agar, 

 gave for some time only the conidial stage with and without setae. This 

 tendency of developing only the conidial stage was lost by continuous 

 sub-culturing on glucose-meat-extract-agar, and that of forming the 



