256 Glomerella ciiiu'uljita mid its Conidial Fonns 



•o 



by small raised dots or pinheads ; this crust was the stroma in which were 

 wholly or partly immersed perithecia. In later subcultures, the pink 

 colour of the young mycelium was completely absent. 



A second lot of cultures from the above five series was at the same 

 time incubated at a temperature of 89-6° F. The growth in all these 

 cultures was again identical but different from that described above. On 

 the upper drier part of the medium it was fluffy, but on the rest of the 

 slant the growth was slightly aerial and the aerial mycelium had formed 

 white feathery strands. The colour of the slant was distinctly pink, re- 

 lieved occasionally by appressed black strands or black dots, which were 

 immature perithecia. On the sides of the medium there was a develop- 

 ment of black stroma which contained acervuli without setae, conidia 

 were also produced on the tips of hyphae but in no part of the cultures 

 were setae developed. The acervuli and conidia were similar to those of 

 G. piperatum E. and E. 



In cultures on corn meal at first the conidial stage was developed 

 which was later replaced by the perithecial; all these cultures were 

 identical in appearance but the one originally started from a single seta 

 formed setae in some of the acervuli which were produced in large 

 numbers while in the other cultures acervuli were few and without 

 setae. 



The perithecia and asci on the host and in cultures are exactly similar 

 to those already described. They show the same range of variation as 

 regards the size and hairiness of the perithecial neck and the size of the 

 asci as that shown by the perithecia and asci of Glomerella cingulata 

 (Stoneman) Spauld. and v. Sch., the periect atage oi Gloeosporimn pipera- 

 tum. The asci (Fig. 14) from the host measure 44-0-82-5 x 9-9-12-1/4, 

 and the ascospores measure 15-4-19'8 x 5-5-6-6)m. The asci are slightly 

 bigger than those generally found on the chilli fruits infected by 

 G. piperatum but they are of the same size as those found on a fruit last 

 year (cf. Fig. 4). The bigger size of the asci is due to the disposition of the 

 ascospores in a single row one below the other. 



The ascospores in size and shape are similar to those already described ; 

 in old perithecia the ascospores have been found to have become septate 

 and to have coloured brown. 



The fungus grows luxuriantly in winter at room temperature — 

 62° to 69° F. — and develops perithecia but the ascospores do not 

 germinate either in water or in nutrient media at this temperature; 

 however they readily do so when incul>;it(Hl at 89-(r F. 



Witli the dcveloj)ment of the peritliecial stage, Glonicrclla ciiiynhild, 



