Jehangir Fardunji Dastijr 257 



from Colletotrichum nigrum E. and Hals, the life-cycle of G. cingulata 

 has been completed. We have seen that 



(1) Glomerella cingulata has been produced in cultures of Gloeo- 

 sporium piperatum E. and E. on partly sterilized chilli stems. 



(2) Acervuli with and without setae, i.e. Colletotrichum, and Glceo- 

 sporium forms, and the perithecial stage have been formed in cultures 

 started originally from a perithecium from a chilli fruit which developed 

 the perfect stage after it had first been attacked by Gloeosporium pipera- 

 tum. 



(3) Acervuli without setae and the perithecial stage, identical 

 with Glomerella cingulata, have been formed in cultures started from 

 (a) a single conidium of Colletotrichum nigrum E. and Hals, and (6) a 

 single ascospore from the perithecial stage developed after incubating 

 the chilK fruit attacked by G. nigrum; and acervuli with and without 

 setae and perithecia have been produced in cultures started from a single 

 seta of C. nigrum. Therefore, now there seems to be no doubt that 

 G. piperatum and C. nigrum are forms of the same fungus and that the 

 ascogenous stage is Glomerella cingulata (Stoneman) Spauld. and v. Sch. 



That Colletotrichum and Gloeosporium on chillies are one and the same 

 fungus is also seen from the study of the conidial forms of the Glomerella 

 on Carica papaya, which is identical with the chilli Glomerella. Single 

 spore cultures started from Gloeosporium and Colletotrichum acervuli 

 have given identical growth ; in both these cultures the perithecial stage 

 and the conidial without setae were developed. In subsequent sub- 

 cultures the conidial stage was lost. Cultures started from single asci 

 and single ascospores have produced only the perithecial stage, as in 

 the case of the chilli Glomerella. 



Variability of the fungus. 



The strains obtained from fruits attacked by Gloeosporium piperatum 

 E. and E. have been cultivated on various media and under varying 

 conditions for months on end but very great or sudden variations have 

 never been observed. As already stated, by continuous subculturing 

 on glucose-meat-extract-agar the conidia bearing faculty was gradually 

 lost and the cultures became sterile. But if these sterile cultures were 

 transferred to sterilized chilli stems, the conidia forming capacity was 

 regained and in subsequent transfers on glucose-meat-extract-agar 

 conidia were formed for two or three successive generations after which 

 the fungus again became sterile. It was however found that this process 

 of getting the conidial stage could not be carried on indefinitely. The 



