Jehangir Fardunji Dastur :259 



greenish black stromatic substratum formed on the surface of the medium. 

 Subsequent subcultures on glucose-meat-extract-agar remained sterile 

 — these cultures were kept running for over a year; but in April, 1918, 

 subcultures on sterilized chilli stems and on corn-meal-agar perithecia 

 were developed but not acervuli. A subculture on glucose-meat-extract- 

 agar from the perithecia- bearing culture on sterilized chilli stems gave 

 only the conidial stage without setae; but transfers on glucose-meat- 

 extract-agar from the perithecial culture on corn-meal-agar were sterile. 

 From this sterile culture transfers were made in May, 1918, on a liquid 

 medium containing (NH)4N03 (1 per cent.), KH2(PO)4 (-5 per cent.), 

 NaCl (-6 per cent.), MgS04 (-25 per cent.), traces of FeClg, CaClg and 

 MnS04 and Glucose (3 per cent.) in 100 c.c. of water. On the surface of 

 the liquid a thick crust pink and black in colour was formed; no fructi- 

 fication was produced. A subculture from this on glucose-meat-extract- 

 agar produced pink acervuli. Setae were exceedingly rare. A culture 

 grown on glucose-meat-extract-agar started from the sterile culture on 

 the same medium, two months old, on the 8th May produced acervuli 

 with setae embedded in a dark black stroma. 



Onbrinjals inoculated in June, 1918, with the sterile culture perithecia 

 were produced. Subcultures on corn-meal in April, 1919, from the sterile 

 culture on glucose-meat-extract-agar which was kept running for a year, 

 acervuli with and without setae were found but no perithecia. In sub- 

 cultures on glucose-meat-extract-agar from the culture on corn-meal in 

 April, 1919, acervuli with and without setae were produced. On corn- 

 meal-agar subculture made in June, 1919, gave acervuli with and without 

 setae. 



From one of the perithecia-bearing cultures of GlCg subculture was 

 made on bouillon agar on the 21st February, 1918: the resulting growth 

 was sterile. From this sterile growth a transfer was made on glucose- 

 meat-extract-agar. The growth of the fungus in this subculture was 

 identical with that of the sterile fungus in the original culture A. In 

 subsequent transfers on glucose-meat-extract-agar the fungus was sterile 

 and formed matted hyphae on the surface of the medium ; greenish black 

 strands of hyphae, especially near about the inoculum, were developed. 

 On chilli seedlings aseptically grown in tubes on moist cotton plugs 

 inoculated with this sterile fungus perithecia were produced. On corn- 

 meal-agar inoculated on the 24th April, 1918, perithecia and acervuli 

 without setae were developed, while on sterilized chilH stems inoculated 

 at the same time and from the same culture only sterile aerial growth 

 was obtained. Subculture on glucose-meat-extract-agar made on the 7th 



Ann. Biol, vi 18 



