260 (xlomerella cingulata and Its Conldial Forms 



May from the fertile growth on corn-meal-agar was sterile. In a liquid 

 medium containing (NH)4N03 (1 per cent.), KH2(P0)4 (-5 per cent.), 

 MgS04 ('25 per cent.), traces of FeCla and MnS04 and maltose (3 per cent.) 

 in 100 c.c. of water, inoculated with the sterile strain, the growth was 

 sterile but the subculture from this, made on the 2nd May on glucose- 

 meat-extract-agar, gave acervuli without setae. From this fertile strain 

 the next generation on the same medium also developed acervuli 

 without setae, but in the subsequent subcultures no fructification was 

 developed. 



In April, 1919, subcultures on corn-meal from the original sterile 

 culture on glucose-meat-extract-agar which was kept going for a year 

 were sterile but were not in appearance different from the cultures GIC^ 

 and GIC3 of the same date on the same medium. Subcultures in May, 

 1919, on glucose-meat-extract-agar, were sterile: there was stroma 

 formation from which setae were developed but no conidia. On corn- 

 meal-agar acervuli with and without setae were produced. 



GIC3, which was a direct progeny of GICj, continued to develop 

 perithecia longer than its ancestor and it did not lose the perithecial 

 forming capacity on glucose-meat-extract-agar as suddenly as GIC^ 

 and GIC2. In subcultures made in February and March. 1918, |)erithecia 

 were not produced all over the agar medium as in the previous cultures 

 but they were confined to the neighbourhood of the inoculum and their 

 presence was marked by a dendritic growth of the perithecial stroma. 

 As the inoculum was generally placed on the upper and therefore drier 

 part of the -slant, it was supposed that the amount of moisture deter- 

 mined the perithecial formation. To verify this supposition some slants 

 were inoculated on the lower moist part and some on the drier upper 

 part. One set of these inoculations were placed in a moist chamber in 

 order to keep the moisture even and a duplicate set which served as a 

 check was kept under ordinary conditions. The growth of the perithecia 

 in both these sets continued to be poor and to be confined to near about 

 the inoculum. In the subculture on glucose-meat-extract-agar made in 

 the beginning of April, 1918, the ])erithecia were absent and the growth 

 was sterile. But in the subculture nuide from this sterile race on the 

 24th of the same month there was a copious formation of acervuli 

 with and without setae, but there were no ])orithecia. The next generation 

 from this acervuli-producing fungus was sterile though it had all the 

 appearance of the culture-bearing perithecia; subsequent subcultures 

 on glucose-meat-extract-agar continued to produce conidia for only a 

 year; setae were absent. The perithecia-forming faculty on this medium 



