Jehangik Fahdunji Dastur 2(i1 



was completely lost. From the sterile culture of April subcultures were 

 grown on corn-meal-agar on the 24th April, 1918. Perithecia were 

 abundantly formed; in the subculture from this perithecial race on 

 glucose-meat-extract-agar the growth was again sterile. Inoculations 

 on sterilized chilli stems and living chilli seedlings aseptically grown in 

 tubes on moist cotton plugs gave a copious crop of perithecia, while on 

 brinjals and peaches only acervuli without setae were developed. Sub- 

 cultures on glucose-meat-extract-agar in May, 1918, from the perithecial 

 strain on sterilized chilli stems gave only the conidial form without 

 setae. 



Subcultures on corn-meal, in April, 1919, from the fungus cultivated 

 for over a year on glucose-meat-extract-agar developed only acervuli 

 with and without setae, and in transfers on glucose-meat-extract-agar 

 acervuli with and without setae were produced but not the perfect form. 

 Subcultures in June, 1919, on corn-meal-agar gave only conidia without 

 setae. 



Inoculation Experiments. 



It seems doubtful if inoculation experiments are of much value in 

 establishing the relationship of the chilli Glomerella with the other species 

 of this genus or in finding the range of hosts of the chilli fungus, as the 

 success or failure of inoculations depends on so many factors, all of which 

 may not be controllable. Different strains or races vary not a little in 

 virulence, e.g., three strains isolated from a culture, originally started 

 by planting perithecia on a nutrient medium, viz., (1) conidial, (2) sterile, 

 (3) perithecial, showed distinct variations in their effects on chilli seedlings 

 grown aseptically on moist cotton plugs in tubes. The perithecial race 

 killed the seedlings in a fortnight and the sterile acted more slowly ; and 

 in both cases perithecia were developed on the inoculated seedlings; the 

 conidial strain, however, infected the seedlings very slightly and no 

 fructification was produced, even in three weeks. But the strain taken 

 in culture from diseased fruits giving only the Glceos'porium stage, which 

 develops in nutrient media only this conidial form, has more virulent 

 infecting power than the strain got from perithecia. Not only is there 

 variation in the virulence of the different strains but there is also varia- 

 tion in the susceptibility of the host, e.g. inoculations on banana fruits 

 have given varying results, some fruits being more readily infected than 

 others. Conditions of temperature and moisture also play an important 

 part in the successful inoculation of the host. Again, in the experiments 

 iu which fruits were used for the inoculation experiments it was at times 



18—2 



