2(32 Glomcrella cingiil;it;i and its ConicUal Forms 



difficult to decide if the fungus was growing on the inoculated host as a 

 parasite or on account of its loss of vitality the fruit merely served as a 

 nutrient medium for the saprophytic growth of the fungus, e.g. the 

 Glceosporium strain was inoculated on apples through a puncture. In 

 four days a slight brown depression round the inoculum was visible but 

 the infection showed signs of increasing only after 25 days and a few- 

 days later the major part of the fruit was sunken and brown, the diseased 

 area showed acervuli of the Glceosporium type. These results of the 

 inoculation can be interpreted in two ways, (1) the infection had really 

 occurred as was shown by the brown depression, but the fungus did not 

 develop further at the time for lack of proper temperature or moisture, 

 (2) the slight brown depression was due to the death of the cells as a 

 result of the puncture and the hyphae had entered the cells after they 

 were dead, and only when the vitality of the fruit was impaired by long 

 storage was the fungus able to grow further. 



This great variability in virulence is found not only in the Glomerella 

 of the chilli but it seems to be a characteristic of this genus, judging 

 from the researches of Barrus^ and Shear and Wood^. 



The results of the inoculations are given below. In all these experi- 

 ments conidia or mycelium from the cultures giving only the Glwo- 

 sporium stage and originally started from a single spore taken from a 

 Glceosporium acervulus from a diseased chilli fruit were used, unless 

 where otherwise stated. 



Chillies. 



Inoculations on fruits have been made on various occasions and the 

 results have not been uniform. Woiind inoculations take more readily 

 than surface inoculations and fruits almost ripe or wholly ripe are more 

 susceptible than unripe fruits. The local varieties are more susceptible 

 than the Peshawar variety. As a rule, in a couple of days after the 

 inoculation of the fruit with the Glceosporium form, a black depression 

 is produced round the inoculum which increases in size concentrically. 

 At first pink acervuli are formed in the black depression but later they 

 appear even beyond the discoloured area. Sometimes there is no ex- 

 ternal sign of the inoculation having succeeded till small raised points 

 appear on the red surface of the fruit. These raised points are the im- 

 mature acervuli. In some cases the infection remained very localised. 



' Barnia, M. F. Variation of \'arietios of Beans in their Susceptibility to Anthracnose. 

 Phytopathology, i. No. 6, p. 195, 1911. 



* Shear, C. L. and Wood, A. K. Studies of Fungous Parasites belonging to the CJcnus 

 Glomcrella. U.S. Dcpt. of Agr., Bui. PI. hid., Bvll, No. 252, p. 74, 1913. 



