Jehangir Fardunji Dastitr 26^i 



When the ])eritliecial form or the sterile and the conidial strains (forming 

 acervuli with and without setae) developed from the perithecial form 

 were used for inoculating fruits the infection took very slowly. At times 

 immature perithecia or sclerotia were developed a week or ten days after 

 the fruits were inoculated; acervuli with and without setae were formed 

 only in isolated cases. At times the infection remained confined to a 

 very limited area round the inoculum. In some cases where the infection 

 had spread almost over the whole fruit (as was evident from the dis- 

 coloration of the skin and the seeds and the presence of hyphae in the 

 loculi of the fruits) still no sort of fructification was developed. In some 

 cases the inoculations had completely failed. Even in the same set of 

 experiments there were varying degrees of the extent of the infection. 



Microtome section of inoculated chilli fruits show that the hyphae or 

 germ-tube can penetrate unbroken cuticle. An appressorium is formed 

 at the tips of an hypha or germ-tube. This appressorium lies closely 

 appressed to the cuticle and puts forth a very fine process or germ-tube 

 which is capable of piercing the cuticle. The process within the cuticle 

 either remain fine or becomes swollen (Fig. 1). 



Inoculations on growing points of mature plants and flower-buds and 

 flowers were tried only with the strain giving the Glceosporium stage; 

 growing points were only slightly infected and that too only under 

 extremely moist conditions. Flower-buds and flowers were readily 

 infected but the infection did not spread beyond the flower-stalk : acervuli 

 were formed in a few days after inoculation. 



Inoculations on seedlings grown aseptically on sterilized cotton plugs 

 have already been described above. 



Carica papaya. 



Flowers and very small fruits, those that have not turned green, 

 take the infection very readily. The first sign of successful infection on 

 the flower is evident by the browning of the tissues in the neighbourhood 

 of the inoculum. This browning extends very rapidly and the whole 

 flower is soon covered with Glceosporium, acervuli. On the fruits which 

 are whitish yellow in colour or are just beginning to turn yellow success- 

 ful inoculations have been secured through the scars of the fallen floral 

 leaves or through the decaying styles. At the point of inoculation a 

 circular depression is noticeable in twenty-four hours. This depression 

 extends concentrically and in three or four days the whole fruit is 

 infected. Fruits that have just turned green take the infection only 

 through wounds, and that too not very readily. From the infected tissues 



