TRICHOMONAD FLAGELLATES. 



299 



On treatment with neutral red the contents stain very differently 

 in different individuals and sometimes differentially from different 

 individual hosts. In some cases (Fig. D, 1) the contents of all of the 

 vacuoles stain red at once. In others, especially in those in which the 



Figure D. Trichomonas augusta Alexeieff after treatment. X1500. 

 Stained granules shown in black . 



1. After prolonged treatment with neutral red giving diffuse staining of 

 vacuoles, probably moribund. 



2. After treatment with methylene blue N showing granules in cytoplasm. 



3. After treatment with neutral red. Vacuoles showing progressive 

 changes in contents and size indicating progress in digestion. 



4. Nucleus and extranuclear motor organelles persisting after maceration 

 of cytoplasm in intestinal mucus, showing rhizoplast connecting blepharoplast 

 and nucleus. 



