TRICHOMONAD FLAGELLATES. 313 



of the stain. Kuczynski (1914) holds that it Hes between the anterior 

 €nds of two "fibrillae" which constitute the axostyle. The figure 

 cited in support (his pi. 16, fig. 101) shows in our opinion the edge of 

 the cytostome rather than fibrillae about the blepharoplast. 



In our figures the blepharoplast is large prior (PI. 1, Fig. 4) to the 

 formation of the intranuclear cloud and small thereafter (PI. 1, P'igs. 

 2, 3, 5). It is a single mass except in cases where mitosis is impending 

 or in progress. From it spring directly without an;v' interruption in 

 structure or stainability the chromatic margin and the chromatic 

 basal rod of the undulating membrane. It is also directly connected 

 with the axostyle and the three anterior flagella. 



In macerated individuals (Fig. D, 4) a delicate strand, the rhizo- 

 plast {rh., Fig. B) may be seen to connect it with the nuclear mem- 

 brane. This is the least chromatic part of the extranuclear apparatus 

 and we have been able to demonstrate it only in macerating individuals. 

 The rhizoplast described by Bensen (1909) in T. vaginalis at the head 

 of a so-called axostyle, is, together with this axostyle, in reality the 

 chromatic basal rod in our opinion, and is extranuclear. 



The extranuclear apparatus, largely chromatic in nature, consisting 

 of the blepharoplast and the organs above named which are connected 

 with it, constitute a structurally connected imit, the extranuclear 

 chromatic motor apparatus. 



The cytoplasmic chromidia {cyt. chr., Fig. B) lie in the cytoplasm 

 between the vacuoles. In size, form and stainability they are like 

 the axostylar chromidia. We find no evidence of their passage from 

 the axostyle into the cytoplasm. They are not always present, being 

 absent in many vegetative individuals (PI. 1, Figs. 1^) and most 

 abundant prior to and during mitosis (Pis. 2, 3), appearing in the 

 cytoplasm with the extranuclear chromidial cloud (PI. 1, Figs. 5, 9). 



The vegetative phases of Trichomonas augusta are found in the 

 intestinal contents and in cultures. The different phases of mitosis 

 may be found in large numbers in smears made from the intestinal 

 wall directly. We will now proceed to follow the process of division. 



This is of two distinct types, binary and multiple fission and both 

 occur in this species and in trichomonads generally on the intestinal 

 wall and may be followed in smears from that region. We are not at 

 present able to relate either process to any definite phase of sexual 

 reproduction. We find as yet no conclusive evidence that either leads 

 to gamete formation by maturation divisions, or that either follows 

 zygote formation or fertilization. Detection of these processes must 

 await, it seems, the unravelling of the history of the true trichomonad 

 cysts. 



