TRICHOMONAD FLAGELLATES. 317 



which one of the daughter membranes has been detached in making 

 the smear. The two membranes are complete even to the posterior 

 flageUum but the new chromatic basal rod has not as yet made its 

 appearance. 



The chromatic basal rod does not originate as does the marginal 

 flagellum by splitting but grows out distally from the blepharoplast 

 along the base of the divided undulating membrane (PI. 2, Figs. 11-18) 

 and ultimately comes to lie (Figs. 17, 19) in the base of one of the 

 membranes. In some cases the two rods appear to be united distally 

 as though they too originated by splitting. If this be the case it is 

 possible that the detachment of the short rod from the long one of 

 Figures 11 to 18 is the result of disturbance of relations in making the 

 preparations, but the mass of evidence is overwhelmingly in favor of 

 the detached outgrowth of the new rod. 



When the two rods or parabasals are completed, the two undulating 

 membranes become more or less widely detached distally (PI. 2, Fig. 

 17), preserving their original connection only in the blepha^'oplast. 

 With the division (PI. 2, Fig. 17) and the migration of the daughter 

 blepharoplasts to the poles of the spindle (PI. 2, Figs. 19-23) the two 

 undulating membrane complexes are finally detached one from the 

 other, save by the connecting paradesmose. In some instances there 

 is a marked disturbance of the normal relation seen in Figures 2U and 

 22, in that the daughter blepharoplasts (PI. 2, Fig. 19) or basal granules 

 after the division of the blepharoplast into basal granule and centro- 

 some, are more or less widely detached from polar relations to the 

 spindle (Fig. 21). We are unable to determine whether this results 

 from the protean activity of the organism in this period or from the 

 disturbance in making smears. There is no evidence in the prepara- 

 tions of mutilation of the body in these cases of disturbed relations. 



The anterior flag ella are produced not by division but by outgrowth 

 of new flagella from the blepharoplast. These sprouting flagella are 

 difficult to detect and are probably o\'erlooked in a number of our 

 figures. They do not all arise at the same time but apparently in 

 sequence during mitosis. The first one appears in the prophase 

 (PI. 1, Figs. 7-11, PI. 2, Figs. 12, 15, 19). When the blepharoplast 

 divides (Fig. 19) one daughter takes two of the old flagella and the 

 other the one and the new flagellum. Another new flagellum is then 

 grown out from each daughter blepharoplast making tliree flagella 

 arising from each, the normal equipment of the vegetative phase. 

 In Figure 19 a second new flagellum is sprouting from the blepharo- 

 plast at the left, but not as yet from that at the right. The full 



