TRICHOMONAD FLAGELLATES. 327 



asexual phase at least) with any karyosome of a hypothetical amphi- 

 nucleolus. Nor is their second conclusion that "Die vermutlich 

 mit (lem Centriol in Zusaminenhang stehende Rippe (Achsenstab) ist 

 eine Art von Centralspindel und geht in die Rippe des Tochterthieres 

 iiber" supported by our observations. 



Dobell (1909) in both Trichomonas batrachorum and Trichomastix 

 bafrachorum finds that the parent axostyle vanishes apparently by 

 absorption and that the daughter axostyles are re-formed out of "the 

 central spindle or centrodesmus" between the daughter blepharoplasts. 

 His figures are inconclusive at the critical stage, however. 



Prowazek and Beaurepaire Aragao (1909) state that in Trichomo- 

 nas columharum the axostyle disappears in greater part, and that 

 "aus dem basalen Teil des Belpharoplastachsenstabsapparates wurd 

 durch die Teilung ein neuer Achsenstab gleichsam aus gesponnen." 



MacKinnon (1910) finds that in the division of Trichomonas trichop- 

 terar the axostyle disappears and is replaced in the daughter cells 

 by the thread connecting the dividing basal granules. Fuller data 

 regarding the axostyle and the fate of the paradesmose in this form are 

 necessary before a critical estimate of the data from this species as to 

 the axostyle can be made. 



Alexeiefl" (1910) states that in Trichomastix motellac the axostyle is 

 resorbed at division and that the new axostyles are formed from the 

 "fusorial band" (centrodesmose) between the daughter blepharoplast, 

 but he figures no stages in this process. 



Janicki (1910) finds in the highly differentiated trichonymphid 

 flagellate Lophomonas blattarum an axial structure which he regards 

 as homologous with the axostyle of the trichomonad flagellates. It 

 persists till late stages of binary fission and of multiple mitosis, and 

 the new organs are formed from the " central spindle" or paradesmose. 

 The evidence is very convincing although there is in the phase corre- 

 sponding to that of axostylar division in Trichomonas, a stage repre- 

 sented in his figures 12-14 in which more data are needed on the 

 precise behavior of the old axial organ and of the paradesmose as it is 

 transformed into the daughter axostyles. It is desirable that these 

 stages be further examined. 



If his account is the correct one, as the evidence in hand indicates, 

 it is directly contradictory to that presented by us from Trichomonas. 

 Three possibilities occur to us to explain this contradiction. The first 

 is that further study will show that the axostyle of Lophomomis also 

 divides as the paradesmose fades out and that Janicki has overlooked 

 this division. The evidence at present in hand is strongly against 



