TRICHOMONAD FLAGELLATES. 347 



forms (PI. 7, Fig. 79) to 10 /x in stouter ones (PI. 7, Fig. 81), is usually 

 7-8 /x, and may attain 12-13 ix in metaphase and multiple fission stages. 



The organs of this flagellate consist of three anteriorly directed 

 flagella, one longer posteriorly directed one, all originating in an ante- 

 riorly located blepharoplast. Adjacent to this organ is the crescentie 

 cytostome, and the spheroidal or ellipsoidal nucleus, and from it 

 passes posteriorly the slender axial axostyle. 



The enveloping cytoplasm has no structurally differentiated pellicle 

 and is not separated into ectoplasm and endoplasm. It is very com- 

 pletely vacuolated with fluid filled vacuoles which are subject to con- 

 siderable variation in size and uniformity. In some individuals they 

 are few and large, four across the body, while in others they are small 

 and numerous six to eight across the body, while in still others they 

 are of mingled large and small sizes in varying proportions. They are 

 usually small and nmnerous in premitotic and mitotic phases. In life 

 and in Schaudinn-iron-haematoxylin preparations these vacuoles 

 show no structural contents and are apparently fluid filled. Over- 

 heated slides show granular contents similar to those found in cyto- 

 plasmic vacuoles of Trichomonas augusta which stain with neutral red. 



There is no evidence with the stains we have employed of solid food 

 particles in these vacuoles, nor has the ingestion of such particles been 

 observed in living individuals. The presence of the cytostome sug- 

 gests its possibility but no evidence that solid particles are ever taken 

 was seen among many living and prepared individuals under observa- 

 tion. No traces of deeply staining chromidia and no well-developed 

 intra- or extranuclear chromidial clouds were detected. The dark 

 granules of uneven size noted by Dobell (1907) do not have the appear- 

 ance of chromidia. Similar granules appear in several of our figures 

 (PI. 7, Figs. 87, 92). The cytostome is a somewhat crescentie tract 

 on the " ventral " side of the body near the anterior end. Its concavity 

 lies adjacent to the nucleus and its upper end is slightly larger. There 

 is no undulating membrane in it and no evidence of a localized pharynx 

 leading from it deeper into the cytoplasm. 



The extranuclear motor apparatus consists of the blepharoplast, 

 flagella and axostyle, all connected and all involved with the nucleus 

 in the process of mitosis The blepharoplast lies rather close to the 

 surface near the anterior end of the body, closely attached to the head 

 of the axostyle. It is of a dense black color, has no cytoplasmic halo 

 about it, is sometimes quadrangular, but usually spheroidal. We have 

 been unable to resolve it into the four basal granules of the four fla- 

 gella, as Martin and Robertson (1911) have for their Trichomonas 



