368 



KOFOID AND SWEZY. 



PLATE 3. 



All figures of Trichomonas augusta Alexeieff , in anaphase (Figs. 24, 25) and 

 telophase (Figs. 26-35) of mitosis, including division of the axostyle (Figs. 

 31-35), unless otherwise stated. X 2175. All from Die»njctylus torosus. 



Figure 24. Late anaphase. Chromosomes elongating and attaching 

 themselves by stainable threads to the poles of the nucleus; 

 blepharoplast at the left near the under surface divided into 

 basal granule and centrosome, its undulating membrane 

 shown in dotted lines; nucleus elongating. 



Figure 25. Latest anaphase in Trichomonas hatrachorum Perty without 

 axostylar chromidia. Chromosomes fusing into several large 

 masses, nuclear membrane constricting, blepharoplasts no 

 longer divided, axostyle very faint. 



Figure 26. Nuclei severed. Chromosomes still intact, grouped about 

 polar centrosome or blepharoplast which in the lower nucleus 

 is detached from the nuclear membrane and carries with it 

 the extranuclear motor complex, blepharoplasts still united 

 by the paradesmose. 



Figure 27. Same stage but blepharoplasts in place, and paradesmose very 

 faint. 



Figure 28. Same stage with paradesmose taut and basal granule detached 

 from centrosome in lower nucleus, whose motor organelles 

 are on the under surf ace ; chromosomes still intact. 



Figure 29. Paradesmose taut, fading out. Blepharoplasts not divided, 

 axostyle related (?) to one; chromosomes still evident. 



Figure 30. Paradesmose bowed. Chromosomes detaching from blepharo- 

 plasts. 



Figure 31. Chromosomes detaching from blepharoplasts Paradesmose 

 no longer visible; axostyle dividing at capitulum, note en- 

 larged axostylar chromidia. 



Figure 32. Tip of axostyle split. Paradesmose in vertical position between 

 blepharoplasts; lower chromatic basal rod represented by 

 dotted line; two nuclei each with single karyosome below the 

 lobes of the axostyle; a second cytostome on the lower surface. 



Figure 33. Axostyle more deeply cleft, one nucleus with single karyosome 

 close to each; the two chromatic basal rods parallel, the lower 

 represented by a dotted line; vacuoles with rod-like (bacteria?) 

 contents. 



Figure 34. Axostyle more deeply cleft, second blepharoplast below and to 

 the right. Note suggestion of division of axostylar chromi- 

 dia. 



Figure 35. Nucleus, blepharoplast and axostyle in approximately then- 

 normal relations in the trophozoite. Axostyle cleft nearly 

 to tip. 



