662 VERHOEFF AND BELL. 



•lowing carbol-thionin solution devised by one of us retains its prop- 

 erties indefinitely and from it a powerful staining solution may be 

 made at once by the simple addition of water: 



Thionin to saturation, about .3 gm. 

 Absolute alcohol, (>0 cc. 



Phenol crystals (melted) 30 cc. 



For use, add one full drop of this solution to 2 cc. of distilled water. 

 Sections are stained as follows : 



(1) Lugol's solution 1:2:100, 1 minute, followed by water, 9»/o 

 alcohol and sodium hx'posulphite solution, to remove mercurial 

 precipitates. Water. 



(2) 0.2% solution of water soluble eosin in 80% alcohol, 5 mmutes. 



Water. 



(3) Carbol thionin diluted immediately before use as above, 5 min- 

 utes. Water. 



(4) Differentiate and dehydrate in 95% alcohol, two changes, until 

 excess of thionin is removed and sections show well marked 

 eosin stain, about 30 seconds. 



(5) Oil of origanum. 



(6) Place on slide, blot, wash in xylol, blot, xylol-balsam. 



To obtain the most brilliant results it is important not to overstain 

 the sections in thionin solution as it is then impossible to produce 

 sharp differentiation of the Nissl bodies by treating with alcohol. 

 The results also are more brilliant the shorter the time that has 

 elapsed between the fixation of the tissues and the staining of the 

 sections. 



The Character of the Reactions of the Ocular Tissues to 

 Abiotic Radiations. 



conjunctiva and cornea. 



Clmical: Our experiments show that the effects on the conjunctiva 

 and cornea of moderate exposures to waves less than 295 /xM m length 

 do not differ qualitatively in their clinical aspects from those produced 

 by longer exposures to waves from 295 MM to 305 mm m length. Severe 

 exposures, however, produce markedly different effects on the cornea 

 in the case of the short waves than in the case of the longer waves 

 owing to the fact that the latter are not fully absorbed by the corneal 

 stroma. The effects of severe exposures to very short waves is there- 



