416 A Tomato Canker 



D. 



14. 4. 20. Six young walnut trees planted in separate tins were numbered (1-6) 

 and inoculated with the six organisms tested in experiments A-C. 



19. 4. 20. Numerous minute water-soaked spots on young leaves on tree (3) which 

 was inoculated with B. Juglandis. 



5. 5. 20. Spots on tree (3) have discoloured and assumed typical appearance of 

 walnut blight lesions. All other trees remained sound and showed no sign of infection. 



E. 



14. 4. 20. Six tins each containing four well-grown cabbage seedlings were used ; 

 the tins were numbered (1-6), the four plants in each being inoculated with one 

 of the same six organisms. 



24. 4. 20. There is an indication of blackening of veins at edges of some of the 

 leaves in tin ( 1 ), plants inoculated with B. campestre. 



12. 5. 20. Cabbage plants in tin (1) show typical black rot lesions. No sign of 

 infection in any of the other tins (2-6). 



F. 



14. 4. 20. Four bean seedlings were inoculated with each of the six organisms. 

 Tins again numbered (1-6), four seedlings in each, and inoculated in the same sequence 

 as in experiment A. 



19. 4. 20. A few water-soaked areas visible on leaves of plants in tin (4) inoculated 

 with B. Phaseoli. 



20. 4. 20. Very numerous points of infection now visible on plants in tin (4). 

 30. 4. 20. Leaves of bean plants in tin (4) now heavily infected and showing 



numerous typical bean blight lesions. No sign of infection on plants in tins (1-3) 

 and (5-6) inoculated with other organisms. 



Inoculation on to other Hosts. 

 A. 



7. 4. 20. 12 tobacco seedlings about six inches high were atomised with a culture 

 of the tomato organism. 



13. 4. 20. No sign of infection. 



7. 5. 20. Still no sign of infection — plants discarded. 



B. 



7. 4. 20. Two plants of Physalis minima were inoculated by atomising with a 

 suspension of a potato culture. 



13. 4. 20. Numerous minute water-soaked spots observed on younger leaves. 



17. 4. 20. Spots have increased in size, are somewhat angular, and are becoming 

 brown. Control plants show no sign of infection. Re-isolated organism from infected 

 leaves and found it to be identical with the original. 



c. 



7. 4. 20. One plant of Datura stramonium var. tatula was inoculated by atomising 

 with a suspension of a culture of the tomato organism. 



