422 A Tomato Canker 



Carrot. On steamed carrot the organism produces in three days a 

 spreading, wet-shining, cream-coloured growth, practically covering the 

 medium, and a thick shiny growth on the surface of the liquid at the 

 bottom of the tube; the colour deepened after the first few days, be- 

 coming straw yellow to amber yellow. 



Turnip. On steamed turnip cylinders growth is similar to that on 

 carrot. The cylinder is entirely covered after three days with wet- 

 shining, slimy-looking growth, which may be flat and smooth or some- 

 what raised, standing out on the surface in large drops. The colour is 

 cream to straw yellow. 



Parsnip. On steamed cylinders of parsnip, growth is similar to that 

 on other vegetable cylinders described but rather less copious. The 

 colour is straw yellow to amber yellow. 



Beet. A very copious, spreading, wet-shining growth is also obtained 

 on steamed cylinders of beet; the growth is more raised than on the 

 other media and straw yellow in colour. On all steamed vegetable 

 cylinders the amount of growth varies with the amount- of moisture 

 present, the best growth being obtained with the maximum amount of 

 moisture. 



Steamed rice. The rice grains become thinly covered with a spreading 

 wet-shining growth which is straw yellow on the wetter parts of the 

 medium and becomes light ochraceous buff where there is less 

 moisture. 



Sweet potato was a very favourable medium, growth being similar 

 to that on the ordinary potato tubes. 



Streak cultures on Loeffler's blood serum. Growth was fairly abundant, 

 in the form of a wet-shining, cream-coloured streak along the needle 

 track; liquefaction was observed on the sixth day and proceeded 

 slowly. 



Nutrient bouillon +15. Growth in bouillon varies considerably with 

 the reaction of the medium. In tubes with a reaction of + 15 of Fuller's 

 scale the bouillon is faintly clouded after 48 hours; it never becomes 

 turbid. A straw yellow ring 1-2 mm. broad forms above the liquid and 

 there are often minute, whitish flocculent particles suspended in the 

 liquid which eventually sink and form a sediment. In + 20 bouillon 

 there is a slight pellicle formed, which sinks when disturbed, and con- 

 siderable sediment is produced. 



Durham's solution becomes lightly clouded. There is no ring, but 

 often a few very minute flocculi in suspension which eventually sink 

 and form a slight sediment. 



