424 A Tomato Canker 



Ammonia by distillation. 



From these figures it will be seen that the amount of ammonia 

 increases steadily up to the 20th day. Similar results were obtained from 

 other tests carried out on the same lines. 



The amount of amino-acids was estimated by the Sorensen method; 

 it increases up to the 15th day and then decreases, the amino-acids 

 being probably broken down to ammonia. 



Amount of N/10 NaOH 

 used for final titration 



In egg albumen medium (1 gm. egg albumen in 50 c.c. of 0-5 per cent, 

 potassium phosphate) similar results were obtained. The qualitative test 

 showed that less tyrosin was formed than in the milk cultures, but a 

 strong reaction for peptone was obtained all through. 



The culture and control were tested for amino-acids and ammonia 

 in the same way as the milk flasks. To an additional flask, zinc sulphate 

 was added to complete saturation, and the total nitrogen in the filtrate 

 determined by the Kjeldahl process; this gave nitrogen as peptone 

 plus amino-acids and ammonia, and by subtraction nitrogen as peptone. 

 It will not be necessary to give detailed figures as in the previous 

 schedules; taking the amount of nitrogen in 1 gm. egg albumen to be 

 •15 gm. a typical test gave the following figures. In each case the control 

 was tested and figures obtained from control flasks deducted. 



Percentage of egg albumen reduced. 



