SOIL PROTOZOA IN SOUTH AFRICA. 377 



Rhizopoda. — .4 moebd proteus. 



Mastigophora. — Peranema trichophorum, Euglena viridis. 



Infusoria. — Pleuronjema chrysalis, Gyclidium glaucoma, 



Oxytricha pellionella, Euplotes liar pa, Paramoecium 



aurelia. 



Rosebank Experimental Station. — The soil was cultivated 

 black, sandy, Liesbeek alluvial loam, collected on 21st June, 1921, 

 in fine cold weather, and was a mixed sample of all the layers 

 from the surface to one foot deep. It gave a feebly acid reaction. 

 In water culture the Protozoa obtained were: — 



Rhizopoda. — Amoeba, proteus, A. radiosa. 

 Heliozoa. — A ctinophrys sol. 

 Mastigophora. — Cercomonas crassicauda. 

 No Ciliata were obtained in this culture. 



Bellville. — The sample was virgin sandy soil collected on 11th 

 January, 1921, at a depth of five to six inches, during slight rain. 

 It gave an acid reaction to litmus. The following Protozoa were 

 obtained from it in water cuiture : — 



Rhizopoda. — Amoeba radiosa, Amoeba spp., Difflugia 



piriformis. 

 Mastigophora.- — Peranema trichophorum, Euglena viridis, 



Cercomonas crassicauda. 

 Infusoria. — Gyclidium glaucoma, Paramoecium aurelia, 

 Oxytricha pellionella, Euplotes harpa. 



Heathfield. — The sample was taken six inches from the sur- 

 face of uncultivated (but apparently fertile) soil, about thirty 

 feet from the banks of a shallow ditch. The soil was collected on 

 11th October, in fine weather. It was blackish, with a slight 

 mixture of grass roots. Arum lilies were growing in profusion in 

 the vicinity. The reaction of the soil to litmus was alkaline. The 

 following Protozoa were obtained in water culture: — 



Rhizopoda. — Amoeba proteus, A. Umax, A. guttula, A. 

 radiosa, J . verrucosa, Difflugia globxdosa, D. pyri- 

 formis, E ughjpha sp* 

 Heliozoa. — A ctinophrys sol, Acanthocystis aculeata, Nu- 

 clear ia simplex^ (a small form). 

 Mastigophora. — Peranema trichophorum , Euglena viridis, 

 OUcomonas (M'onas) termo, Jiodo (Prowazekia) parva 

 or terricola,\ Pleuromowis jacidans. 

 Infusoria. — C yclidiu in glaucoma, Stylonychia mytilus. 



High Constantia. — The sample was taken at a depth of nine 

 inches fiom the surface, and was a light-brown, readily crumbling 

 soft soil, under cultivation with the best Hermitage grapes, used 



* This species of Euglypha lias not quite the same shell patterning as 

 E alveolata, and somewhat resemhles the form described by Martin 

 and Lewin. 



f This genns is som* times placed with the Proteomyxa. 



X The determination of this S23ec:es is difficult, with the restricted litera- 

 ture at our disposal. It appears to be either B. parva or B. terricola. 



