378 SOIL PROTOZOA IN SOUTH AFRICA. 



for the production of Vlakkenberg wines. The waters in the neigh- 

 bourhood are radio-active. The sample was collected on 14th 

 October, in fine weather. Its reaction to litmus was acid. The 

 water culture showed: — 



Rhizopoda. — Amoeba proteus, Binucleate amoeba cyst. 



Mastigophora. — Oikomonas termo, Bodo (Prowazekia) 

 parva, Euglena viridis, Pleuromonas jaculans. 



Infusoria. — Cyclidium glaucoma, Lacrymaria olor.. 



The organisms in this culture were always very few in number. 

 It is possible that the known radio-active character of the water of 

 the locality has some action inimical to Protozoa. 



Simonstown. — The specimen was collected from uncultivated 

 soil at Seaforth. Simonstown, during fine, hot weather, on 8th 

 January. The soil was brownish and sandy, about fifty feet 

 above sea level. The sample was taken from a depth of about six 

 inches. Its reaction to litmus was acid. Ericas were growing 

 near by. Water cultures yielded the following Protozoa: — 



Rhizopoda. — Amoeba Umax, A. radiosa, A. guttula, Dif- 

 flugia globulosa, Hyalosphenia elegans, Euglypha 

 sp. 



Heliozoa. — Actinophrys sol, Aca/nthocystis aculeata. 



Mastigophora. — Mastigamoeba sp., Perdnema tricho- 

 phorum, Oikomonas (Monas) termo, Bodo (Prowa- 

 zekia) parva, Euglena viridis, Pleuromonas jaculans. 



Infusoria. — Cyclidium glaucoma, Lacrymaria olor. 

 A few Vibrios were found in this culture, and Mastigamoebae 

 containing ingested bacteria were also observed. 



Stellenbosch. — The specimen of soil was collected from a fur- 

 row in a newly-ploughed and planted vineyard, about six inches 

 from the surface. It was a sandy, greyish-red soil, with a few 

 shreds of plant roots, and was obtained on 12th October in fine 

 warm weather. It gave an acid reaction to litmus. The following 

 Protozoa were obtained in water culture: — 



Rhizopoda. — Amoeba proteus. A. Umax, A. guttula, A. 

 verrucosa, Pelomyxa. palustris, Difflugia globulosa, 

 D. pyriformis, Hyalosphenia elegans, Euglypha 

 alveolata. Euglypha sp. 

 Heliozoa. — .4 ctinophrys sol. 



Mastigophora. — Mastigamoeba sp., Peranema tricho- 

 phorum, Oikomonas (Monas) termo, Bodo (Prowa- 

 zekia) parva, Euglena viridis, Pleuromonas jaculans. 

 Infusoria. — Cyclidium glaucoma, Colpoda cucullus, Lacry- 

 maria olor, Paramoecium aurelia, Stylonychia mitylus. 



Cultures of this soil also contained a few bacteria, mostly 

 vibrios, and specimens of Colpoda cucullus and of Pelomyxa were 

 found containing ingested vibrios 



Kimberley. — This specimen was a reddish-brown sandy loam 

 and was collected from an orange grove on 31st July, in fairly 



