SOIL PROTOZOA IN SOUTH AFRICA. 385 



Euplotes patella, found at Umgeni, Cedara (black vlei). 

 Vorticella campanula, found at St. James, Umgeni, May- 

 ville. 



(B) Results of Examinations of Waterlogged Soils. 



Examinations of waterlogged soils were not possible until the 

 onset of the rainy season (November) in the Transvaal, and hence 

 the samples are few, but these have been added to the paper for 

 the sake of completeness. All the waterlogged soils were feeblv 

 acid in reaction. 



Five samples of waterlogged soil were investigated by direct 

 examination in order to ascertain whether trophic Protozoa 

 occurred therein. In each case the sample of soil was placed in 

 a beaker and allowed to stand for about an hour under a bell jar. 

 The liquid that oozed from the soil was withdrawn in a sterile 

 pipette and examined. 



Specimen 1. — Johannesburg garden soil was taken from a 

 shallow ditch that cairied off storm water and waste water from 

 two dripping taps. It was said to be always waterlogged. A 

 specimen was collected on 1st December, 1921, when about an inch 

 of storm water was above its surface. 



The following trophic Protozoa were counted in one-tenth c.c. 

 of ooze : — 



Rhizopoda. — 2 A moeba radiosa- 



Mastigophora. — 14 Euglena viridis, 20 Oihomonas termo, 



15 Bodo (Broirazekia) parra, some of which contained 



ingested bacteria. 



Infusoria. — 1 Colpidium striatum, 3 Holophrya orum. 

 Specimen 2. — This soil was collected in Johannesburg from 

 the side of a land drain similar to the preceding one, but though 

 the soil was very damp, there was no standing water. The speci- 

 men was obtained on 12th December, and one-tenth c.c. of the ooze 

 contained the following organisms: — 



Rhizopoda. — 1 .1 mocha radiosa. 



Mastigophora. — 13 Euglena viridis, 22 Oihomonas termo, 



15 Bodo (Prowazekid) parra. 

 Infusoria — 3 Holophrya orum. 



Specimen 3. — This specimen was collected on 13th December, 

 from the waterlogged bank of a shallow ditch draining the veget- 

 able garden of the Fort, Johannesburg. Heavy rain had fallen 

 two days previously. The following Protozoa were obtained in 

 one-fifth c.c. of ooze: — 



Rhizopoda. — 1 A mocha proteus, 1 A moeha radiosa. 

 Mastigophora. — 5 Euglena viridis, 2 Bodo parva. 

 Infusoria. — 2 Holophrya ovum, 12 Coleps hirtiis, S 

 Cyclidium glaucoma, 2 Vorticella campanula. 



Specimen 4 was a sample of waterlogged soil from the banks- 

 of the Sans Souci Dam, between three and four miles from Johan- 



