PUCCINIA DIGKAPHIDIS. 215 



spores from which were ripe and germinating on May 27th. No 

 result followed on Allium. 



2. May 5th. — With artificially produced teleutospores and 

 promycelium-spores from Phalaris, which were in a state of germi- 

 nation, I infected P. arundinaeea, ConvaUaria, Allium, Arum macu- 

 latum, Polygonatum multiflorum, and Lilium bulhiferum. 



Spermogonia appeared on ConvaUaria on May 14th, and beyond 

 slight yellowish spots appearing on Polygonatum, not the least 

 result followed on Phalaris, Allium, Arum, or Lilium. 



3. May 12th. — Orchis mascula, Gagea lutea, Allium Scorodo- 

 prasum, A. ursinum, Scilla nutans, Polygonatum officinale, P. multi- 

 florum, Arum maculatum, ConvaUaria majalis, and Phalaris arundi- 

 naeea, were infected with germinating teleutospores and promy- 

 celium-spores from Bowness, with the result that spermogonia were 

 developed on ConvaUaria on May 20th, and were succeeded by 

 fficidiuin cups on June 6th. No result ensued on Allium, Arum, or 

 any of the other species. 



4. May 22nd. — The last-mentioned experiment was repeated, 

 additional plants of ConvaUaria being used, with the same results. 

 Spermogonia were conspicuous by June 1st on ConvaUaria, but no 

 result whatever on the other plants. 



5. May 27th. — Germinating aacidiospores from ConvaUaria were 

 applied to the leaves of other plants of the same species. No 

 results. 



6. May 31st. — Phalaris arundinaeea was infected with artificially 

 produced ascidiospores from ConvaUaria majalis. Eesult : on June 

 18th red spots of uredo were conspicuous on the leaves. 



7. — Three plants of ConvaUaria from Bowness, infected by the 

 ascidiuru, were planted on June 13th, 1889, and kept isolated. 

 This year the plants are healthy, and up to the present time 

 (June 19) are entirely free. 



Mr. Plowright* has also produced the ascidium on ConvaUaria, at 

 King's Lynn, from teleutospores on Phalaris from Bowness. 



Puccinia digraphidis, n. sp. 



1. (jEcidium ConvallarUc). — Pseudoperidia mostly hypophyllous, 

 seated on whitish or yellowish circular spots, circulating, or in 

 small clusters, more elongated and irregular on the petiole, shortly 

 cylindrical or nearly flat, margins white, torn. Spores sub-globose, 

 epispore minutely verrucose, contents orange, 15-30 x 14-22 jx. 

 On ConvaUaria majalis. 



2. Sori on yellowish spots, small, reddish brown, subrotund or 

 linear, sometimes confluent, scattered or collected in groups, 

 erumpent. Spores subglobose or elliptical, epispore finely punc- 

 tate, orange-red. 20-30 /v.. in diameter. On Phalaris arundinaeea. 



3. Sori numerous, persistent, black, covered by epidermis, at 

 first small, then linear, confluent, forming lines 1 to 3 mm. long 

 on the leaves, longer on the sheaths. Spores brownish, smooth, 

 very irregular, oblong, wedge-shaped, curved or elongate, apex of 



* Gard. Chron. May 24th, 1890, p 643. 



