242 Journal of Agricultural Research voi. xvii, No. 5 



its normal size. On cross section of the vesicle numerous hemorrhagic 

 areas were observed, as well as a number of necrotic centers, the latter 

 being confined chiefly to the more central portions of the organ. So 

 softened were some of these foci that the necrotic material assumed 

 a semifluid character. The capsule of the organ showed considerable 

 thickening. (See PI. 22.) 



HisToi^OGiCAL EXAMINATION. — Sections from the left seminal vesicle 

 showed varying stages of the diseased process, ranging from exfoliation 

 of the epithelial lining of a few of the acini to complete obliteration 

 of the normal glandular structure. There was marked proliferation of 

 the interstitial tissue with round cell infiltration, which was more pro- 

 nounced immediately surrounding the acini and just beneath the 

 epithelial lining of the acini. In those areas exhibiting the more pro- 

 nounced pathological changes many of the acini were filled with detached 

 epithelial cells and cell debris. In other areas where the mere outline 

 of the acini could be traced, a homogeneous substance was present, 

 together with more or less granular detritus. There were hemorrhages 

 into and between the acini. Occasionally large areas of degeneration 

 and necrosis were observed. As a result of the inflammatory changes 

 little normal glandular structure was recognized in many of the sections 

 examined (PI. 24, A and B). Plate 23 A, representing a normal seminal 

 vesicle, is inserted for comparison with Plate 23 B, and Plate 24, A 

 and B. 



BacterioIvOgical findings. — Eighteen tubes of medium were utilized 

 for the culturing of the different organs. The six tubes from the left semi- 

 nal vesicle after three days' incubation developed from 75 to 1 50 colonies 

 of an organism that appeared typical of Bad. abortus. All tubes inocu- 

 lated from other sources remained sterile, although incubated for several 

 additional days. Subsequent work with the organism isolated estab- 

 lished its identity as Bact. abortus and indicated that pure cultures of the 

 organism were isolated in all instances. 



EXAMINATION AND FINDINGS OF BULL 1 36 



May 15, 1918. Agglutination test: 0.04 cc.-H, 0.02 cc.-|-, o.oi cc.^-, 

 0.005 cc. SI. 



May 16, 1918. Slaughtered. 



Macroscopic examination. — The right seminal vesicle showed slight 

 enlargement. The fluid contained by this organ presented a turbid 

 appearance. No lesions were elsewhere detected. 



BacteriologicaIv findings. — Five of the i8 tubes of medium inocu- 

 lated from the different organs developed from 40 to 60 colonies of an 

 organism that was subsequently identified as Bad. abortus. These inoc- 

 ulations were from the right seminal vesicle. 



