Journal of Applied Microscopy. 247 



Paramecium, 



Drawing. — Whole animal, showing shape of body, cilia, vestibule, food glob- 

 ules, non-contracting vacuoles, contracting vacuoles, nucleus, trichocysts. x 

 about 200. 



Observations on the Living Atiimal. — 1. Is the protoplasm in the body of Para- 

 mecium quiet or moving ? 2. Place Paramecium in a drop of gelatin solution 

 (2.5 per cent, gelatin in water). Cover with a cover-glass, select a quiet individ- 

 ual, and observe how the carmine grains pass by it. Indicate by arrows placed 

 outside the periphery of the drawing the direction of movement of the carmine. 

 What do you infer concerning the movement of the cilia ? Do the grains whirl 

 as much about a moving individual as about a quiet one ? Can you explain ? 

 Why are not all the Paramecia carried off by the current when the carmine is run 

 under the cover-glass? 3. Place upon a glass slide a drop of water con- 

 taining Paramecia. When they are uniformly distributed, put a few grains of 

 common salt as the margin of the drop. After a few seconds observe the distri- 

 bution of the organisms. How is the result to be interpreted ? 4. Place upon the 

 glass slide a drop of water containing Paramecia ; alongside, but not in contact, 

 place a drop of beef extract. Connect the two drops by a thread of water. After 

 a few seconds observe the distribution of the organisms. How is the result to 

 be interpreted ? 



Topics for the Teacher. — 1. How the Infusoria get into the aquarium? 2. 

 The habitat and food of Paramecium. 3. Allies of Paramecium; Stentor, 

 Vorticella, Carchesium, Euglina, the Suctoria, Amoeba, the Gregarinidae. 4. 

 Economic importance of Amoeba and the Gregarinidae. 5. Reproduction of 

 Paramecium. 



Butterfly. 



Drawings. — 1. Imago, dorsal view, wings expanded, x 1 or 2. 2. Imago, 

 left side, wings closed. (The bodies in one and two are to be drawn parallel to 

 each other.) x 1 or 2. 3. Imago, front of head, x 10. 4. Pupa, left side. 

 5. Full grown larva, dorsal view ; full grown larva, left side. 



Questions on External Anatomy. — 1. How many segments behind the head in 

 {a) the imago ? (J)) the larva ? {c) the pupa ? 2. What external organs of the 

 imago can be identified in the pupa ? 3. Which feet of the larva correspond to 

 those of the imago ? 



Observations on the Living Larva. — Each student (or group of students) 

 should be provided with a glass vessel covered with netting and containing food 

 leaves, for keeping the larva during pupation. 1. How is locomotion effected? 

 Illustrate by diagrams. 2. How does the larva feed ? Observe and record the 

 movements of the mouth parts and of the head during feeding. Draw the outline 

 of a partly eaten leaf. 3. (This observation must extend through several days.) 

 Make and record observations upon the act of pupation. 



Topics for the Teacher. — 1. The habits and food of butterflies. 2. The num- 

 ber of broods of butterflies during a single season, and seasonal dimorphism. 

 3. Protective resemblance and mimicry. 4. The larger divisions and commoner 

 native forms of Lepidoptera. (Examples of Lepidoptera illustrating the common 



