258 Journal of Applied Microscopy. 



noticeable impression of your finger on it. Too niucli albumen will ruin the 

 preparation. The albumen fixative is made as follows : 



1. 25 cc. of the white of a fresh hen's egg. 



2. 25 cc. of glycerin. 



3. 0.5 gram sodium salicylate. 



Shake Avell and filter. This will keep well for at least six months. 



2. Now lay the slide down on the table and put a few drops of distilled 

 water on it, on top of the albumen film. Care must be taken here that the 

 water will not flow over the edge of the slide. 



8. Cut the ribbon into suitable lengths, according to the size of the square 

 or oblong cover-glass, discarding the ends of the ribbon which do not contain 

 sections. With a scalpel lay the pieces of ribbon on the water in the center of 

 the slide in such a manner that one may begin at the upper left-hand corner 

 and follow the sections in lines, as one reads the words on this page. Allow- 

 ance must always be made for a certain amount of stretching of the ribbons 

 when they are heated, as they are always more or less ruffled. Never press the 

 sections down with the finger or by any other means, else the fine structure will 

 be broken and distorted. 



4. Warm the slide gently by putting it on the paraffin oven until the heat 

 has straightened out the sections on the water, but do not let the sections get so 

 hot as to melt the paraffin. The slides may now be placed on wooden blocks, 

 which may be kept constantly on top of the oven for this purpose. It is best to 

 let them remain for about twelve hours, when the water will all be evaporated 

 and the sections firmly dried to the slide. Four, eight, or more slides can be 

 carried through at one time just as well as a single one. 



9.— STAINING. 

 The sections are now ready for the staining. One must have the following 



Stender dishes : 



1. Filled with turpentine. 



2. Filled with xylol. 



o. Filled with absolute alcohol. 



4. Filled with 95 per cent, alcohol. 



5. Filled with 85 per cent, alcohol. 

 G. Filled with 70 per cent, alcohol. 



■ 7. Filled with 50 per cent, alcohol. 



8. Filled with 25 per cent, alcohol. 



9. Filled with distilled \vater. 



The various stains used may also be kept in Stender dishes if no special stain- 

 ing dishes are at hand. The following stains are recommended for general 

 purposes : 



1. A////iu safranin, alcoholic (50 per cent.) solution, made by combining 

 equal parts of anilin water and a saturated alcoholic (95 per cent.) solution of 

 safranin. The anilin water is prepared by shaking up anilin oil in distilled 

 water. About 3.5 per cent, of anilin oil will be taken up by the water. 



2. Gentian i-iolcf, a 2 per cent, aqueous solution. 



