Journal of Applied Microscopy. 



349 



rabbits three, eight, and twenty-eight days. The most favorable time to determine 

 nerve centers by this method was in from fourteen to eighteen days after the 

 section of the nerve. The tissues were hardened in part, in alcoliol, to be used 

 after in Nissl-stain, and part in Marchi's fluid. In one case the spinal cord was 

 put in a 2.5 per cent, solution of formalin, and single pieces either in alcohol 

 according to Nissl, or left for a short time in Miiller's fluid, and stained according 

 to Marchi. To stain the ganglion cells, Nissl's methylen-blue method was used, or 

 the same author's magenta red and Senhossik's thionin. The last method gave 

 very good results, but methylen blue was best for the finest changes. a. m. c. 



Jacottet, G. fitude sur les alterations des 

 cellules nerveuses de la moelle et des gang 

 lions spinaux dans quelques intoxications 

 experimentales. Beitr. zur path. Anat. u. zur 

 allgem. Path. 22 : No. 3, pp. 443-465, q fig. 

 Abstr. Zeitsch. f. wiss Mikros. IScj; 1897. 



The author applies Nissl's method 

 with modifications of Schaffer, Pandi, 

 Sarbo, Sadowsky ; the last being es- 

 pecially useful. This method is to 

 harden pieces of fresh cord in an 

 aqueous 10 per cent, solution of formalin for two to four days ; putting it in 95 per 

 cent, alcohol for forty-eight hours, and finally in absolute alcohol for an indefinite 

 time. If sufficiently hard, pieces are cut directly or in celloidin. Sections are 

 stained one to two minutes in a concentrated solution of fuchsin in 5 per cent, 

 carbolic acid aqueous solution. After staining, sections are bleached in an acetic 

 acid solution till the differentiation between the white and gray matter is clear ; 

 further differentiation occurs in the absolute alcohol, and sections are cleared in 

 xylol and mounted in balsam. Sadowsky used methylen blue instead of fuchsin, 

 a freshly prepared 5 per cent, solution, in which the slides remain for half an hour. 

 Ramon y Cajal's excellent method was used : passing sections direct from 95 

 per cent, alcohol in concentrated aqueous solution of thionin for a few minutes, 

 and then into a mixture of equal parts of anilin and absolute alcohol ; when the 

 sections only showed a light blue they were put in xylol and then balsam. These 

 methods, however, do not produce permanent stains. a. m. c. 



Coles, A. C. The Blood : How to Examine and 

 Diagnose its Diseases. London (Churchill), 

 1898. 8; 60 plates. 



Ogneff, J. Ueber die Entwicklung des elek- 

 trischen Organs bei Torpedo. Arch. f. Anat. 

 u. Phys. Phys. Abth. p. 270, 1897. Abstr. 

 Zeit. f. wiss. Mikro. 15: 1898. 



Jolly. Recherches sur la valeur Morphologique 

 et la Signification des Differents Types de 

 Globules blanc. Theses. Paris, 1898. 



Birmingham, A. A Study of the Arrangment of 

 the Muscular Fibers at the Upper End of the 

 Esophagus. 5 fig. Transact, of the R. Acad, 

 of Med. of Ireland. 16: pp. 422-431 and 432- 

 440. 



Brouha, M. Recherches sur le Developement 

 du Joie, du Pancreas de la clison mesente- 

 rique et des Cavites hepato-enterique chez 

 les Oiseaux. 3 taf u. 20 fig. Journ. de I'Anat. 

 et Phys. T. 34 ; 305-363. 



A Perforated Swimming Porcelain Cup for Washing 

 Out Specimens. - — The accompanying illustration shows a 

 porcelain cup which is perforated and is closed by a cork. By 

 the aid of the cork the cup swims in the water. It is made 

 for washing out specimens, e. g., pieces which have been 

 decalcified. 



Emil Amberg, M. D., Detroit. Mich. 



