Journal of Applied Microscopy. 377 



6. The bactericidal products of blood serum and of leucocytes cannot be 

 identical. h, h. w. 



Smith, Theobald. One of the Conditions under It was found that shallow layers of 

 which Discontinuous Sterilization may be bouillon, steamed in the Arnold steril- 



Ineffective. Jour. Exp. Med. 3: 647-650, • r - 1 ^i. r 



jg g ' t/ J J2er for an hour, on three or four 



successive days, developed anaerobes 

 after some days' growth of diphtheria bacilli which had formed a membrane over 

 the surface and excluded the oxygen. In other cases, aerobes on solid culture 

 media produced changes, that aroused growth in dormant spores of anaerobes, 

 which apparently do not find conditions favorable for development in the 

 intervals between the heating of the culture medium. To insure perfect steriliza- 

 tion, the culture medium may be heated to a temperature of 110" to 115" C. 

 Where apparatus for heating to such a temperature is not available, the anaerobes 

 may be destroyed by steaming the bouillon three or four times in round liter 

 flasks filled to the neck, and afterward placing the flasks in the incuVjator, where 

 the anaerobes will develop in a couple of days. The bouillon may then be 

 poured or siphoned into sterile culture flasks if it is to be used in shallow layers. 



E. M. Brace. 



Hektoen, Ludvig. The Fate of the Giant Cells Rjts of Sterile solidified blood serum 



which Form in the Absorption of Coagulated . , , . , , , . , , 



Blood Serum in the Anterior Chaniber of were mserted into the anterior chamber 



the Rabbit's Eye. Jour. Exp. Med. 3: of the rabbit's eye, and animals were 



^ -^ ^ ^ ■ killed at intervals of from a few days 



to six weeks after the operation. During the early stages there is a limited 



emigration of polymorphonuclear leucocytes, some of which returned to the iris 



after becoming loaded with granules of serum. New cells are formed which 



accumulate around the foreign body, and most of them take up granules from 



it with their cytoplasm. During this time there are formed multinucleated giant 



cells or plasmodial masses, which appear to result from the fusion of many small 



cells. At first these giant cells are more or less circular, with smooth margins. 



Later, they become oblong in shape and free from granules, but there is no 



degeneration nor any immigration of small cells or leucocytes into the giant 



cells. In one instance a dividing nucleus was found in a giant cell. 



As the serum disappears the giant cells become marked with lines of 

 cleavage which map out spindle-shaped cells. These uninuclear masses, partly 

 detached, as well as small cells resembling them, are found about the giant cells. 

 The writer concludes that giant cells formed under these conditions resolve 

 themselves into small cells which unite with other newly formed cells to form a 

 fibrillated tissue resembling the cornea in structure. He also considers that the 

 results furnish evidence opposed to the general teaching that giant cells of 

 tuberculosis are necrobiotic from the time of their formation. h. h. w. 



Frankland, Percy, Ph. D., F. R. S. The Action Photographic plates were placed about 



of Bacteria on the riiotographic Plate. Cen- 1 if ; j ^ and below gelatin 



tralbl. f. Bakt. 24: 609-612, 1898. . ^ 



cultures of Proteus vulgaris, and 



Bacillus coli communis, to determine what effects might be produced. Both 



cultures gave strong light effects on the sensitive plates over the dishes, but the 



