382 Journal of Applied Microscopy. 



Sodium sulph-indigotate - - - - 0.1 gram. 



Carbolic acid, 5 per cent. Aq. Sol. - - 100 cc. 



This stains the nuclei red, the cell bodies apple-green, and the bacteria pur- 

 ple (if gentian violet was used in the Gram's solution). 



Lithium-carmine-Gram's method : 



Lithium-carmine, 10 minutes. 



Acid alcohol (1 per cent. HCl in 70 per cent, alcohol), 1 minute. 



Pure alcohol, 95 per cent., 8 minutes. 



Proceed with Gram's staining as usual. 



Results — nuclei red, cytoplasm pink, bacteria purple. 



Picro-lithium carmine may be used in the same manner as given above for 

 picro-carmine. 



The following may be used after completion of decoloration in Gram's method : 



Bismarck brown, 2 per cent. aq. sol., 8 minutes. 



Eosin in 70 per cent, alcohol, 1 minute. 



Logwood (Delafield's), 8 minutes. 



Then in acid fuchsin 1 per cent., orange G Sat. Aq. Sol. aa., "2 minutes. 



Logwood (over-stain), 10 minutes. 



Picric acid Sat. Aq. Sol., 100 cc, 5 minutes. 



1 have lately used the following stain, which, so far as I know, is original 

 with me, with very pleasing results, both for celloidin and paraffin sections. The 

 only disadvantage which I can find is in the slightly increased length of time 

 required. 



Stain for 15 minutes in logwood, 12 drops; sulph-indigotate of soda sol., 2 

 drops, Decolorize in nitric acid, 1 per cent., 20 drops. Dehydrate, fix, and 

 darken the stain in alcohol, 95 per cent., 20 drops. 



Next proceed as in the ordinary Gram's staining, but substitute basic fuch- 

 sin for the gentian violet usually employed : 



Anilin water fuchsin, 10 minutes. 



Gram's sol. of iodin, 2 minutes. 



Alcohol, 95 per cent., ^ minute. 



Acid alcohol, 10 seconds. 



Alcohol, 95 per cent., 5 minutes. 



Creasote (best beechwood), 8 minutes. 



Mount in Canada balsam. 



Results — Nuclei deep blue, cytoplasm apple green, bacteria bright cherry red. 

 This method brings out structural detail in the tissue better than any of the bac- 

 terial counter-stains which it has been my good fortune to have opportunity to 

 use. Louis Lerov, M. D. 



Vandeibilt University Medical Dept. 



Abstract of Paper by Dr. B. Meade Bolton, entitled, "The Signifi- 

 cance OF THE Results of Bacteriological Examination for the Diph- 

 theria AND Tubercle Bacilli." — After a brief description of bacteria in 

 general, as to size, shape, method of reproduction, etc., the paper was devoted 

 to the diseases diphtheria and tuberculosis. Statistics show that in Paris, during 



