412 Journal of Applied Microscopy. 



NORMAL AND PATHOLOGICAL HISTOLOGY. 



Richard M. Pearce, M. D. 



Pathological Laboratory, Boston City Hospital, Boston, Mass., to whom all books and papers 

 on these subjects should be sent for review. 



CURRENT LITERATURE. 



Jenner, Dr. L. New Preparation for Rapidly Equal parts of a 1.2 per cent, solution 

 Fixing and Staining Blood. Lancet, I : •570- <■ /-. -i 1 , ^ 111 • ,, 



171 iSqq. of Grublers water-soluble eosin, yellow 



shade, and a 1 per cent, solution of 



Griibler's medicinal methylen-blue, are thoroughly mixed by stirring with a glass 



rod, and filtered after twenty-four hours. The residue is dried at a temperature 



not exceeding 55 degrees C, powdered, washed on a filter with distilled water, 



dried, and again powdered. 



The fixative stain is made by dissolving 0.5 g. of this preparation in 100 cc. 



methyl alcohol. Cover-glass preparations are made by allowing a few drops of 



the solution to act on the dry film for from one to three minutes. Then pour 



off the stain, rinse in distilled water from five to ten seconds, or until the films 



become pink, and dry the cover-glass, preferably in air. E. M. Brace. 



Clark, J. G., M. D. The Origin, Growth, and There are two theories in regard to the 



Fate of the Corpus Luteum, as observed , , , r ,1 1 



in the Ovary of the Pig and Man. John development of the COrpus luteum. 



Hopkins ^Hospital Reports, 7: 4, 1898. According to V. Baer's theory, the one 



Also Archiv. fiir Anat. und Physiol. Anat. , .11-11. 



Abtheilung 1808. more commonly accepted, this body is 



derived from the cells of the theca 

 interna. iYccording to BischofT's theory, the lutein cells are modified cells of 

 the membrana granulosa. 



From the ovary of the pig, Clark first obtained a complete set of specimens 

 representing all changes, from the maturity of the Graafian follicles to complete 

 organization and disappearance of the corpus. With these he compared the 

 changes in the human ovary. 



In order to study the relations of the connective tissue he used a digestion 

 (trypsin) method. The principal stain used was a modification of Van Gieson's 

 picro-fuchsin stain. 



His results agree with those of v. Baer. The lutein cells are specialized 

 connective tissue cells which appear in the follicle wall at the time of its differen- 

 tiation into the theca interna and externa. The corpus luteum is therefore a 

 connective tissue, and not an epithelial, structure. The lutein cells are formed 

 at the expense of the ordinary connective tissue which forms a fine reticular 

 tissue between the cells and also produces the membrana propria. Upon the 

 rupture of the follicle the membrana propria is broken through, in places, by 

 the advancing lutein cells and blood vessels, but quickly reforms a connective 

 tissue line in front of the lutein cells. This is pushed by them to the center of 

 the cavity, where it forms a dense core of interlacing fibers. 



After rupture of the follicle the lutein cells, by increase in size and number, 

 fill the cavity. The degeneration which follows is probably induced by the 



