436 Journal of Applied Microscopy. 



from the side opposite the entrance of the auditory' nerve. Continue sectioning 

 until the scala media is exposed from apex to base of cochlea. 



{e) 7(* per cent, alcohol for four hours. 



(/) Borax carmine for twelve hours. 



(<^) "^^^ P^J" cent, acidulated alcohol until destained. 



(//) 85 per cent, alcohol, three hours. 



( / ) 95 per cent, alcohol, three hours. 



(y) Place material in '1 oz. bottle containing about 2 inches of 95 per cent, 

 alcohol ; add about one-half inches of cedar oil, and allow the material to remain 

 in this mixture until it sinks to the bottom of the bottle (do not shake the bottle). 



{k) Place the material in pure cedar oil for three hours. 



(/) Add to the pure oil, paraffin that has been dissolved in cedar oil ; continue 

 to add little by little until the oil will not dissolve any more paraffin ; keep the 

 bottle in the sun, or in some other warm place for a day or two, adding fresh 

 paraffin for the first day. 



(w) The material now being completely saturated with paraffin, place it in 

 the paraffin bath for one hour, being careful to keep it just at the melting point. 

 Now imbed, being careful to orient it so that the desired view may be obtained. 



When the material has been sectioned, fix the sections, which need not be 

 very thin, on the slides with Mayer's albumen, and allow them to dry. Then 

 place the slides in : 



(a) Xylol (without melting the paraffin) for five minutes. 



(J?) Absolute alcohol for two minutes. 



(r) 95 per cent, alcohol for two minutes. 



{d) 95 per cent, alcoholic solution of picric acid for one minute. 



{e) 95 per cent, alcohol two minutes. 



(_/) Absolute alcohol two minutes. 



{g) Xylol five minutes. 



{h) Mount in balsam. 



Mel T. Cook, Professor. 



H. H. ZiMMERiMANN, Assistant. 



De Pauw University. 



A square of thin mica makes a good cover-glass to use in studying fish'eggs, 

 frog eggs, etc. On account of the flexibility of the mica, the eg^ is less liable to 

 be crushed than with a glass cover, and if the effects of pressure on the develop- 

 ment of the egg are to be studied, the mica cover is easily manipulated. 



A bottle for collecting insects may be made from an olive bottle, or any con- 

 venient wide-mouthed bottle. Place some potassium cyanide in the bottom, 

 cover with plaster of paris, and sprinkle on enough water to wet the surface so 

 that when it dries a hard crust will form over the top. Label poison. Keep the 

 bottle closely corked, and be careful not to breathe the contents. 



