444 Journal of Applied Microscopy. 



In considering the genuine bacteria, he regards the theory that bacteria are 

 nuclei without cytoplasm as unfounded. In well fixed material neither SpiriUum 

 nor other bacteria show clear, less deeply staining ends. The more deeply stain- 

 ing granules are not nuclei or chromatin granules, and present methods fail to 

 demonstrate a nucleus. The relationship of the sulphur bacteria and all other 

 bacteria to the Cyanophyceae is merely one of loose, superficial morphology. 

 They are more closely related to the Flagellatae. 



The technique throughout is thoroughly up to date. Microtome sections from 

 paraffin material were stained in the most approved manner, and the plates are 

 exceptionally elegant and definite in details. c. j. c. 



RECENT LITERATURE. 



Buscalioni, L. Sopra un nuova caso di incap- Perrot, E. Anatomie comparee des Gentiana- 



sulamento dei granuli di aniido. Malpighia, cees. Ann. des Sci. Nat. Bot. Sen VIII, 7: 



13: 3-13, pi. I, 1899. 105-292, pi. 1-9, and 29 figs, in text, 1899. 



Daniel, L. La variation dans la greffeet There- Ricome, H. Recherches experimentale sur la 



dite des caracteres acquis, {fin.) Ann. des sy me trie des rameaux floreaux. Ann. des 



Sci. Nat. Bot. Ser. VIII. 8: 193-226, pi. Sci. Nat. Bot. Ser. VIII, 7: 293-396, pi. 



i-io, 1898. 10-13 and 13 figs, in text, 1899. 



Halsted, B. D. Root Tubercles and Nitrogen Schrenk, H. vou. A disease of Taxodium 



Appropriation. Extract from the 26th Ann. Known as reckiness,also a Similar Disease of 



Rep. of the New Jersey State Board of Libocedrus decurrens. Contributions from 



Agriculture, Jan. 1899. the Shaw School of Botany, No. 14. Report 



Pee=Laby, F. £tude anatomique de la feuille of the Mo. Bot. Garden, 11: 1-55, pi. 1-6, 



des Graminees de la France. Ann. des Sci. 1899. 



Nat. Bot. Ser. VIII, 8: 227-347, pi. 11-13, Voglino, P. Di un nuova malattia dell' Azalea 



1898. indica. Malpighia, 13 : 73-86, pi. 2-3, 1899. 



ANIMAL BIOLOGY. 



Agnes M. Claypole. 



Separates of papers and books on animal biology should be sent for review to 



Agnes M. Claypole, Sage College, 



Ithaca, N. Y. 



CURRENT LITERATURE. 



„ ,. I, „, -, , ^ • ^ r , These ova are remarkably compact in 



Carlier, E. W. Note on the Ovarian Ova of the _ ^ ^ 



Hedgehog. Journ. Anat. and Physiol. 33: form and have peculiarly large nucleoli, 

 304-308, 1899. ^j^^^ hence are favorable for the study 



of these features. The Graafian follicle is early represented as a layer of flattened 

 cells arranged round the ovum ; the nucleolus is strikingly large, about two microns 

 in diameter, and taking a homogeneous stain. As the follicle cells become 

 arranged in a double row, the nucleoli increase in size, and many "yolk" nuclei 

 appear in close contact with the nuclei, but in the cytoplasm. These latter event- 

 ually migrate to the periphery and spread out into a layer of flattened bodies 

 round the margin of the egg. The nucleus by this time shows irregular out- 

 lines and moves from its originally central position. The nucleolus now shows at 

 least one vacuole and measures at least seven microns in diameter. This nucleolus 

 may now be bodily extruded and another form, or vacuolation, may continue until 

 a frothy, spongy mass results, taking the stain but slightly. The author con- 

 cludes that the nucleolus is an accumulation of wa.ste products that is eventually 



