Journal of Applied Microscopy. 447 



Winternitz's method for tubercle. Sections are stained in a two to three per 

 cent, anilin water solution of fuchsin, decolorized in a 50 per cent, alcoholic fluor- 

 escein solution till the sections appear a light rose color, then they are counter- 

 stained with methylen blue. The basophilous granules are most clearly differen- 

 tiated by their red color. a. m. c. 



The author sets forth details of his old 

 Tryaska-Chezonszczewsky. Uebermeine , -i ^ij t u ^-^ 



Methode der physiologischen Injection der but very successful methods of physio- 



Blut und Lymphgef'asse. Virchow's Arch, logical injection, dealing with the pro- 



153: No. I, p. IIO-I2Q, pi. I, i8q8. r i-rr ^ <- 1 



^ ^' F . V cesses for different organs separately. 



1. For the blood vessels of the spleen a neutral ammoniacal cochineal-carmine 

 solution, 10 to 100 cc, according to the size of the animal, is injected into the 

 jugular vein ; after five minutes, before the carmine begins to appear in the urine, 

 the abdominal cavity is opened and the veins, arteries, and the spleen cut out and 

 hardened in 80 per cent, alcohol. After hardening microscopical preparations 

 are made in the usual way and mounted in Canada balsam and Tammar shellac. 



2. For the vessels of the liver a double physiological injection of veins and 

 lymphatics was obtained by injecting indigo-carmine into the blood of the living 

 animal and after ten minutes, before the coloring fluid appeared in the kidneys, 

 cochineal-carmine is injected into the blood. After five minutes the abdominal 

 cavity is quickly opened and the vessels of the liver ligatured. In sections pre- 

 pared from this material blood vessels are stained with cochineal-carmine and 

 round them lymph vessels full of lymph, and stained blue with indigo-carmine. 



3. Lymph vessels of the lungs. The action of carmine toward living lung paren- 

 chyma differs greater from its action toward dead tissue. It stains neither inter- 

 cellular substance nor the cells of the different tissues ; it also leaves unchanged 

 the epithelium of the bronchi with which it comes into undoubted contact. It 

 brings out particularly the characteristics of lymph vessels. The carmine goes 

 from the bronchi to the cells of the inner tissue, through the finest canals 

 of the lung alveoli whose stomates lie between the epithelial cells. After a quar- 

 ter of an hour the urine of the animal is colored red with carmine. The animal 

 is then killed quickly with chloroform and the lungs injected with BerUn blue 

 through the pulmonary artery. The lungs are then taken out and hardened in 

 alcohol or cut frozen. 3. The lymph vessels of the diaphragm. — 10 to 100 cc. of 

 neutral ammoniacal cochineal-carmine solution is injected into the abdominal cavity, 

 the amount varying with the size of the animal. After five to ten minutes, as 

 soon as the urine shows color, the diaphragm is removed and washed in clear 

 water and hardened in 70 per cent, alcohol; then cleared in turpentine or 

 another aetherial oil, and mounted in Canada balsam or Tammar shellac. With 

 the naked eye the injection of the large and medium sized lymphatics can be seen, 

 and under the microscope the very finest. 5. The connective tissue between the 

 blood and lymph capillaries. — An animal is injected with carmine solution through 

 the external jugular vein, and after five minutes the abdominal cavity is opened. 

 Cutting through the lower part of the diaphragm, the flap is turned back and 

 quickly washed, first in pure water and later in a solution of silver nitrate. Then 

 the diaphragm is cut out, put into 80 per cent, and then absolute alcohol, cleared, 



