^148 Journal of Applied Microscopy. 



and mounted. These preparations show clearly the blood vessels carmine 

 stained, and filled with blood, while the large and small lymphatics are brought 

 out by the silver nitrate. Along their whole course one sees clearly a rose car- 

 mine color which is neither in the ground tissue of the diaphragm nor in the 

 connective tissue of the lymph spaces. These spaces are closely pressed into trian- 

 gular forms in the region of the blood capillaries. The inside of these triangular 

 enlargements of the spaces, as also the spaces themselves, are colored with the 

 carmine, while the connective tissues remain unchanged. 6. The lymph vessels 

 of the skin. — The skin is found to be pervious to aqueous solutions and the fol- 

 lowing method is used to demonstrate the course of the lymph vessels. Cochi- 

 neal carmine is used, with this precaution, that there shall be an excess of carmine 

 in the solution to prevent the maceration of the skin by the ammonia. The ani- 

 mal is put into this liquid and the steps of the color can be followed. After three 

 hours, carmine has penetrated the epidermis, later the hair bulbs, the outlets of 

 the sebaceous glands, and the glands themselves. After six hours the coloring 

 has spread to the connective tissue, bringing out the spindle and stellate ele- 

 ments and their processes with great clearness. The lymph vessels form a thick 

 net which rise in the papillary layer in the form of loops, lying below the blood 

 capillary loops. This superficial network passes directly into the deeper and 

 larger lymph vessels. To demonstrate the absorptive powers of the granulation 

 layer, a longitudinal piece of skin was taken from the backs of dogs and other 

 animals, the wound treated in the customary way. After the healing granulation 

 tissue had formed, cochineal-carmine or indigo-carmine solution was dropped upon 

 the wound. In vertical sections through the whole thickness the red or blue 

 stained capillaries were most clearly visible. a. m. c. 



CURRENT BACTERIOLOGICAL LITERATURE. 



H. li. Waite, 

 University of Michigan. 



Separates of papers and books on bacteriology should be sent for review 



to H. H. Waite, 710 East Catherine street, 



Arln Arbor, Michigan. 



Novy, Dr. F. Q. Laboratory Work in Bacteri- Laboratory manuals in bacteriology are 

 ology. University of Michigan. Pp. 563. becoming somewhat common, but Dr- 



George Wahr, Ann Arbor. ,,. . ^ , ^ 1 ^ . • 1 • 



^ Novy IS to be congratulated m havmg 



produced one which will certainly be among the most useful for bacteriological 



students. Although the title indicates that it is a book designed for laboratory 



work, it proves to be more than this, since it gives a brief course in general 



bacteriology. The first five chapters give a general account of bacteria, their 



structure, life history, and relations to the general problems of fermentation. 



This introduction is excellent, and admirably serves to inform the student in 



regard to the general nature of the organisms which the rest of the work gives 



him directions for studying in detail. In choosing the subjects to be treated in 



this brief introduction, Dr. Novy has been successful in selecting topics of real 



