528 Journal of Applied Microscopy. 



^ ., T^ . , . , . , , Keifer has demonstrated, by injection 



Keifer. Etiologie et developpment des myo- ' ^ -> 



mes de I'uterus. Belgique Societe d'anato- methods, small islands of non-vascu- 



mie Pathologique. La Presse Medicale, j^j. ^jg^^jg ^^j^j^,!^ increase peripher- 



No. lO, pp. 49, 1899. . . r r- 



ally by the addition of uterine smooth 

 muscle fibers, and which are afterwards penetrated by vessels which 

 afford them nutrition. These are often found in the zone of tissue supplied 

 by one vessel, or its collateral branches. In the center of these small myomata 

 is often seen an arrangement of fibers, suggesting the lumen of a vessel in which 

 the injection has not penetrated. Keifer believes that uterine myomata arise 

 from these small muscular bodies. This takes place by hypertrophy of these 

 bodies, as the result of their encysting vascular trunks rendered useless by 

 thrombosis, prolonged compression, and other processes interfering with the 

 circulation. r. m. p. 



Ranvier. Histologic de la peau. Definition This investigator divides the skin of 

 et nomenclature des couches de I'epider- , ^, ., . , 



mie Chez I'homme et les mammiferes. "^^n and the mammifera mto seven 

 Acad, des Sci., Paris, LaPresse Medicale, distinct layer, as follows : 1. Ger- 

 • 7. pp- 3 . 99- minating layer, composed of cells in 



which rapid division takes place. 2. The filamentous layer, in which the cell 

 protoplasm has many fibrils (prickle cells). 3. The. granular layer, character- 

 ized by cells with eleidin granules. 4. The intermediate layer, which separates 

 the two principal layers of the skin. 5. The stratum lucidum. 6. The stratum 

 corneum. 7. The stratum disjunctum, or superficial desquamating layer. 

 Each of these layers has perfectly distinct physical and chemical characteristics. 



R. M. p. 



Ranvier. Histologie de la peau. Sur quel- Eleidin is seen in the midst of the 



. ques reactions histochimiques de I'elei- cells of the Stratum granulosum in the 



dine. Acad, des Sci., Paris, La Presse Medi- , ^ , n^, ^ . ,., 



cale, No. 12, pp. 55, 1899. ^^^^ of granules. They stam readily 



with carmine, haematoxylin, and thionin. 

 Lime water does not dissolve them, but renders them more distinct by swelling 

 the cellular protoplasm. In the transformation of the cells of the stratum granu- 

 losum to the stratum intermedium, the eleidin granules disappear, and there is 

 found instead a homogeneous substance, staining still more strongly with 

 carmine. R. m. p. 



Neutral Red. — This is used as an iiitra-vitam stain, and reacts upon the 

 cytoplasmic granules, and contents of mucus cells. Tadpoles placed in a 

 Trioir ^^ To oVo solution for a day or two are stained dark red. 



Rothberger (Centrlbl. f. Bakt. xxii, 515, 1898), uses it to distinguish Typhus 

 from Coli bacilli. The concentrated aqueous solution is transparent, and has no 

 trace of fluoresence, but a coli culture of neutral red agar after twenty-four hours 

 becomes strongly fluorescent, and shows a clearing up of the stain, while a typhus 

 culture remains unchanged. As far as the experiments were carried the reaction 

 seemed to be characteristic for coli. The best preparation consists of 10 cc. 

 fluid agar, three or four drops concentrated aqueous solution of neutral red, and 

 about one-half cc. of a twenty-four hour coli bouillon. The fluorescence also 

 shows well in gelatin cultures, but in these, large brownish-red crystals are formed. 

 Other references: Zeits. f. wiss. Mik. xi, 2, '250, 1899. Galeotte, id. p. 198, 



