536 Journal of Applied Microscopy. 



Turner, Mt. Holyoke College ; Elizabeth W. Towle, Bryn Mawr College ; John 

 R. Murlin, Univ. of Pennsylvania ; Louise B. Wallace, Smith College ; Anne 

 Moore, Vassar College ; A. P. Hazen, Bryn Mawr College ; Gary N. Calkins, 

 Columbia Univ.; Cornelia M. Clapp, Mt. Holyoke College ; Elliott R. Downing, 

 Brooklyn, N. Y.; Martha Bunting, Columbia Univ.; Edward Rynearson, High 

 School, Pittsburg, Pa.; Mary Alice Wilcox, Wellesley College ; Caroline B. 

 Thompson, Univ. of Pennsylvania; Frederick S. Lee, Columbia Univ.; S.J. 

 Holmes, Ontario, California ; Robert M. Yerkes, Harvard Univ.; Edward Thorn- 

 dike, Columbia Univ.; Oliver S. Strong, Columbia Univ.; E. C. Strobell, N. Y. 

 City; George G. Scott, Williams College; Frederick C. Paulmier, Columbia 

 Univ.; Wales H. Packard, Bradley Poly. Institute ; W. W. Norman, Univ. of 

 Texas ; Margaret L. Nickerson, Univ. of Minnesota ; A. D. Morrill, Hamilton 

 College ; T. H. Morgan, Bryn Mawr College ; Thomas H. Montgomery, Univ. 

 of Pennsylvania ; Albert Mathews, Tufts Med. College ; Elias P. Lyon, Bradley 

 Poljtechnic Institute; James H. McGregor, Columbia Univ.; Wm. A. Locy, North- 

 western Univ.; Ralph S. Lillie, Chicago Univ.; Arthur E. Hunt, Manual Training 

 High School, Brooklyn, N. Y.; Louise H. Snowden, Univ. of Pennsylvania. 



The Laboratory of the University of the Pacific. — The laboratory 

 IS arranged so that the light comes from one direction, it is heated with steam 

 and has direct ventilation. Common tables, about 3 x 5, are placed two at 

 a window, and one table is usually assigned to two students, each having his 

 own private drawer. Microtome knives, scissors, scalpels, forceps, and other 

 dissecting instruments, slides, cover-glasses, etc., are furnished by the student. 

 'There is a laboratory fee, sufficient to cover the expense of whatever course is 

 -chosen, and when this is paid the student is given the required apparatus, which 

 may be returned at the end of the year, when the money will be refunded for all 

 that is uninjured. Reagents and stains are also provided by the laboratory, 

 each student being given a complete set of reagents that are in constant use, 

 such as the different grades of alcohol, clearing fluids, etc. Although there are 

 not as many microscopes as students, the work is arranged so that there are 

 enough to supply all working at any one time. Responsibility for the care of 

 instruments is placed upon the students as far as possible. Each student is 

 required to keep a note-book, in which he records what he gets in the talks, 

 keeps an accurate account of his work, and makes his drawings. The private 

 biological laboratory has water, gas for lighting and heating, a full cabinet of 

 materials, reagents and stains, a microtome and everything necessary for making 

 slides. AH specimens for preservation are usually prepared in this laboratory. 

 University of the Pacific. RUE D. FiSH. 



Stain for Neusser's Perinuclear Basophilic Granules. — 



( Acid fuchsin, 55 cc. 

 Saturated aqueous solution of ■} Orange G, 70 cc. 



( Methyl green, 80 cc. 



Distilled water, 150 cc. 



Absolute alcohol, 80 cc. 



Glycerin, 20 cc. 



"Film preparations of blood stained with this, show in gout, and leukaemia, a 

 grouping of dark, blue-stained granules in uni- and multinucleated leucocytes. 

 Cabot claims that these granules have no clinical significance, and states that he 

 has a triple stain that brings out Neusser's granules in all blood, normal and 

 abnormal. 



