554 



Journal of Applied Microscopy. 



II. SIMPLE APPARATUS FOR FILTERING THROUGH A PASTEUR- 

 CHAMBERLA-ND FILTER. 



The accompanying diagram explains this apparatus better than anything 

 which could be added in the text. For use, the chimney is simply grasped by a 

 good retort clamp, or large size burette holder, so as to 

 steady it and hold it firmly in place. The top of the filter- 

 ing bougie is connected as usual with the flask, in which 

 a vacuum can be made if desired. The bougie, with 'its 

 connections, may be sterilized, as may the lamp chimney. 

 After sterilization the rubber tubing, CC, which may 

 have been disinfected in some chemical solution, or by 

 steam, is slipped over 

 the top of the chimney, 

 the bougie inserted, and 

 the tubing slipped 

 cautiously over the 

 shoulder of the bougie. 

 We do not know whether 

 there is anything spec- 

 ial in the glass of the 

 lamp chimney which 

 renders it unusually re- 

 sistant to changes of 

 temperature, but we 

 would observe that we 

 have never had one of 

 these chimneys break 

 as a result of steriliza- 

 tion. By properly se- 



TUNMEL -.^ 



Ot. BOTTLE. -» 



Figure II. Simple Appar- 

 atus for Filtration through 

 Pasteur-Ch amberland 

 Filter. 

 {Drawn by P . A . Skeaff".) 



B. A chimney of the ordinary 



German student's lamp, chim- lecting the cliimney and 



ney as shown is inverted. AA. " 



Pasteur -Chamberland bougie, boUgie, a very aCCUrate 

 "^ upon the shoulder of which the ° •' 



chimney rests. CC. Piece of £t may bc SCCUred, and 

 heavy rubber tubing which slips ■' 



over the small end of the chim- hcnce Small quantities 

 ney and the shoulder of the 



bougie. of fluid may be filtered 



comparatively rapidly. We are of course per- 

 fectly aware of more elaborate forms of apparatus 

 involving the same principle, but we recommend 

 this by reason of its simplicity, and the fact 

 that it may be improvised almost anywhere. 



III. BATTERY OF JARS FOR WASHING 

 SPECIMENS. 



In some form or another the principle illus- 

 trated in the accompanying drawing has been 

 utilized in our laboratories for a number 'of 

 years. Large specimens, such as lungs, are 



COPPER GAUZE 



TISSUE, WASH\N(;-^ 



DIAGRAM OF BATTERX OF BOTTLES — 



TOR WASHING SPECIMENS IN 

 FLOWING- WATER ARROWS SHOW 

 — COUKSC OT WATC-R 



Figure III. Battery of Jars for 



Washing Specimens. 



{Drawn by P. A. Sheaff.) 



