Journal of Applied Microscopy. 567 



To obtain the blood for determining the numerical relation of plates, the 

 author used small blown glass tubes sealed at both ends ; breaking off an end, 

 the tube is filled from the fresh blood mass and the blood is received into a 

 nearly airless tube. Instead of sealing the tube with heat, sealing wax was used, 

 which was in every way satisfactory, and avoided the injury found to follow the 

 heat required to fuse the glass. Blood so prepared was scarcely coagulated 

 after twenty-four hours, but remained thin and a bright red. a. m. c. 



Muller, Fr. Die morphologischen Verander- The author used for his Studies the 



ungen der Blutkorpcrchen und des Fibrins living blood clotS formed by extravasa- 



bei der vitalen extravascuiaren Gerinnung. ^- • .. r i. u u r ^i r 



Beitr. z. Path. Anat. u. z. allgem. Path, tion m the front chamber of the eye of 



23: 498-528, I pi., 1898. (Abst. in Zeit. the rabbit. By puncturing the cornea 



90-92, I 99.} with a sterile, blunt-angled, sharpened 



canula, an extravasation is obtained. By operating quickly and removing only 



part of the contents, the escape of the humor from the chamber can be prevented, 



and a repetition of the operation in the same eye is rendered possible. The liquid 



in the canula is put on a sterile Hollundermark cover, sealed with vaseline on 



a hollow ground slide, and examined under 800 or 1000 magnification. For 



fixation the covers were either heated on a copper plate, or dried in the air and 



treated with 10 per cent, formalin-alcohol (1 pt. 40 per cent, formaldehyde and 



9 pts. of absolute alcohol). Concentrated sublimate solution, followed by iron 



haematoxylin stain, gave excellent results. Good results, but less certain, were 



obtained by treating with a four per cent, formalin solution, followed by alcohol 



of increasing concentrations. The liquids are allowed to flow drop by drop on 



the cover ; a strong stream must be avoided. These are stained for half an hour 



in Delafield's haematoxylin, and for the same time in picro-fuchsin. Heiden- 



hain's haematoxylin counter-stained with eosin and eosin methylen blue and 



triacid were used more rarely. 



In fresh preparations amoeboid movement could be seen in the leucocytes 

 either with or without the use of neutral red. During coagulation the leucocytes 

 can be seen to send out thick or thin thread-like processes, on which red- 

 dish granules were sometimes observed. As in the ordinary blood clot, the sub- 

 stance, after the destruction of the cell elements, appears as a homogenous mem- 

 brane. The processes from the corpuscles soon become invisible. Shortly 

 after the removal of the liquid from the eye, on the addition of neutral red, a 

 reddish network of threads can be seen forming, enclosiftg the blood plates and 

 corpuscles. Some fibers are especially broad, wavy, and homogenous, and 

 these, after fixation with heat, remain only partially differentiated. 



Similar results were obtained by raising a blood mass in the subcutaneous 

 tissues on the back of a guinea pig. Six to eight Hollundermark covers are 

 placed in the wound and left for three hours. On removal they are fastened 

 together by a firm clot and are treated in a four per cent, f ormol and Miiller-sublimate 

 solution. These show the same network structures. The author concludes that 

 these processes show fiber formation not typical of true fibrin. 



A. M. c. 



