568 Journal of Applied Microscopy. 



CURRENT BACTERIOLOGICAL LITERATURE. 



H. H. Waite, 

 University of Michigan. 



Separates of papers and books on bacteriology should be sent for review 



to H. H. Waite, 709 North University avenue, 



Ann Arbor, Michigan. 



Pfuhl, E. Untersuchungen iiber die Entwick- The colon bacilli used in this investi- 

 elungsfahigkeit der Typhusbacillen auf gation were isolated from the faeces of 



vtrS^nsefn^on Slibacillin uS^^^^ three dififerent individuals, and all were 



ien der Gartenerde. Centrlblt. f. Bakt. 26: shown tO possess no real motion in 

 49-5 1' I 99- ^]^Q hanging drop examination. The 



Eberth bacilli, on the other hand, were all actively motile. Mixed cultures of the 

 colon and Eberth baciUi were made in the following manner on potato. First a 

 small amount from an agar culture of the Eberth bacillus was spread over the 

 cut surface of a cooked potato, and the surface thus covered was then smeared 

 with a colon culture. The mixed culture was placed in a moist chamber and 

 kept in the incubator for seventeen hours. At the end of this time an examina- 

 tion was made of the upper layers of the excellent growth which had developed, 

 but only colon bacilli were found. From the border of the growth material was 

 now taken, the platinum loop being forced into the substance of the potato, and 

 in the hanging drop made from this the Eberth bacilli were found, though few 

 in number. After removing all apparent growth from the surface of the potato, 

 hanging drops were made from the surface thus laid bare, and Eberth 

 bacilH were shown in greater numbers, showing that the germ had penetrated 

 the substance of the potato. Like results were obtained in all of the three 

 Eberth and three colon cultures which were received from different sources. In 

 one instance the cuhures were examined for three successive days, and the 

 Eberth bacillus was present each time. 



Cultures were also made by streaking potatoes first with the Eberth bacillus 

 and then with garden earth. The result was the same here except that it was 

 necessary to isolate the Eberth bacillus by the plate method. From these 

 investigations it is shown that the Eberth bacillus can grow on cooked potato in 

 the presence of colon bacilli and of bacteria of various kinds found in garden 

 soil, and that they penetrate the substance of the potato. h. h. w. 



The results obtained by the investiga- 

 Dinwiddie, R. R. The Relative Virulence for . , . ^^ / ^ ,, 



the Domestic Animals of Human and Bovine tion are briefly summed up as follows : 

 Tubercle. Ark. Agri. Exp. Stat. Bull. No. Cattle— i^m//«<^ fesfs with tubercular 



1:7. June, 1899. . r^r t 1 1 



sputa of mail. — Of four calves sub- 

 jected to this test all failed to give a tuberculin reaction, and, according to the 

 post mortem examination, all remained absolutely uninfected. 



Inoculation of sputum and bovijie tubercular material. — Intra-tracheal inocu- 

 lation was tested on two calves. The sputum-injected calf alone responded to 

 the tuberculin test. The changes found on post mortem were, in both cases, 

 slightly roughened patches on the pleura, without visible evidence of any tuber- 



