Journal of Applied Microscopy. 569 



cular process ; in the sputum test no local lesion, in the other a number of 

 abcesses in which tubercle bacilli were found. 



Intra-peritoneal inoculation; four calves in each series. Of the sputum 

 series, two gave doubtful and two negative reactions to tuberculin ; of the 

 bovine series, two positive and two negative. The pathological changes in the 

 sputum lot were : in one no lesions, in another a few roughened patches and 

 seed-like granules on the omentum, with slight fringe formation on various parts 

 of the abdominal serosa, a few whitish spots on surface of liver. The fourth 

 was not examined post mortem. 



The bovine series showed: in one no lesions, in two changes similar to 

 the above but somewhat more extensive ; in the fourth a very extensive develop- 

 ment of grape-like growths, containing tubercles all over the peritoneum, a few 

 of same on thoracic surface of diaphragm, tuberculosis of some of the abdominal 

 lymph glands. 



Comparative tests tvith sp2itu7n and bovhie cultures. — Intra-tracheal inocula- 

 tion on two calves. The sputum-tested animal gave no reaction to tuberculin. 

 The post mortem findings were slight pleuritic adhesions, fringe development 

 on costal pleura, a few lymphoid nodules of the size of millet seed histologically 

 showing no relation to tubercles in lungs. Bovine culture : locally a number of 

 abscesses external to treachea, and fungus-like ulcerating growths on its mucous 

 surfaces, all containing tubercle bacilli ; lower cervical lymph nodes tubercular. 



Intra-peritoneal inoculation : two calves, sputum culture. Reaction to tuber- 

 culin. On omentum are several patches studded with millet-seed-like calcareous 

 granules, and one small bean-sized polypoid growth; a few mesenteric glands, 

 tubercular. Bovine culture : fatal result in six weeks. Acute tubercular 

 disease involving all the abdominal and thoracic organs ; lesions in the form of 

 miliary tubercles, large yellow nodules and fibrous grape-like growths on serous 

 surfaces; -tubercle bacilli very abundant. 



Inoculation in lung : two calves, sputum culture. No tuberculin reaction ; 

 no post mortem. Bovine culture : tuberculin reaction ; abscess with surround- 

 ing consolidation and tubercles in lung ; numerous tubercles in pleura ; fringe 

 formation ; tuberculous bronchial glands. 



Feeding : two calves ; sputum culture. No reaction to tuberculin ; no 

 post mortem. Bovine culture : reaction to tuberculin ; mesenteric glands 

 tubercular, many yellow spots on liver. 



Vic's,.— Feeding sputa to six pigs resulted in infection in two, the disease 

 being confined to the submaxillary and mesenteric glands. 



Intra-peritoneal inoculation of sputa and bovine tubercular material.— Yowx 

 pigs ; sputum lot. One died from other disease before effects could be pro- 

 duced. The other shows a local lesion, a general tuberculosis of abdominal 

 serosa, tuberculous abdominal and thoracic lymph glands, and embolic tubercles 

 in lungs. Bovine lot : both presented essentially the same condition as the 

 last described, in the abdominal cavity, but no extension to thorax. 



Intra-peritoneal inoculation of cultures. — Two pigs ; sputum culture. No 

 lesions. Bovine culture : in one generalized, in the other localized in sub- 

 maxillary and mesenteric glands. 



