570 Journal of Applied Microscopy. 



Sheep — Intra-peritoneal inoculation of ciilUires. — Two sheep ; sputum cul- 

 ture. Patch of seed-like granules on omentum. Bovine culture : generalized 

 disease, ending fatally. 



Chickens- — Feeding sputa. — Six chickens, all unaffected. 



Intra-abdominal inoculation of sputum and bovine cultures. — Ten chickens. 

 Both lots were unaffected. 



A comparison of the virulence for cattle of human and bovine tubercular 

 material seems to show that the bovine material is more virulent. For pigs 

 there seems to be no difference in virulence, both being capable of producing 

 infection. Sheep are highly susceptible to bovine tubercle bacilli. The 

 results in regard to human tubercle bacilli were not sufficiently worked out to 

 arrive at a definite conclusion. Chickens were very insusceptible to both. It 

 also seems that there can be no doubt that cattle are susceptible to infection 

 from human tubercular material, and that the disease is similar to the naturally 

 acquired tuberculosis of cattle, though the lesions are usually much less exten- 

 sive. All comparative tests, with the exception of that of Chauveau, show a 

 greater susceptibility of cattle for bovine than for human tubercular material. 



H. H. w. 



Beco, L. Note sur la valeur de agglutination The author has tested the action of 

 par le serum antityphique experimental .... , ^ i- 



comme moyen de diagnostic entre le baciUe anti-typhoid serum and formalm on 

 d'Eberth at les races coliformes. Centrlblt. cultures of the Eberth and colon ba- 

 f. Bakt. 26: n,6-n,q, i8qq. .,,.,.,. 



cilli obtamed from various sources, 



and has drawn the following conclusions from his observations : Agglutination 

 caused by an experimental antityphic serum is a valuable and practical means for 

 the differentiation of the Eberth-Gaffky bacillus, provided that the serum used is 

 very active. The test should only be considered positive when agglutination 

 takes place with a dilution considerably less than the dilution with which the 

 most sensitive of the colon group responds to the reaction. Antityphic serum 

 is endowed with a very variable power of agglutination with respect to the colon 

 bacillus. In some cultures apparently morphologically and biologically identical 

 there is no reaction, in others it is considerable. Formalin does not agglutinate 

 certain well established typhoid cultures. On the other hand, it frequently 

 causes the agglutination of the colon bacillus and other bacteria often met with 

 in the stools and in water. The conclusions drawn from these facts is that the 

 agglutinating property of formalin is of interest theoretically rather than practi- 

 cally. H. H. w. 



Epstein, S. Apparat zum sterilen Abfi.llen The apparatus consists of a vessel, R, 



von Fliissigkeiten. Centrlblt. f. Bakt. 26: for the sterile fluid, a graduated bur- 



ite. abt., 34-35, 1800. .. T- 1 • 1 r- • 1 11 • T^ , 



-^ ^^ ette, E,which fits mto a bell-jar, F, closed 



below with a cork upon which is placed a thin layer of wool. The glass vessel 

 R is made broad so as to do away with as much height as possible, and is open 

 both above and below. The upper opening of R is wide and closed with cotton. 

 To prevent evaporation a ground-glass cap with a spiral glass rod which is closed 

 above with cotton, was used (Fig. 2). The lower opening of R is drawn out and 

 serves as a means for connecting the glass vessel R with the burette E. 



