584 Journal of Applied Microscopy. 



b. Place in a saturated aqueous solution of picric acid for four days. 



c. Leave in a 5 per cent, aqueous solution of bichromate of ammonium for 

 from four to six days in an incubator, or from three to four weeks at room tem- 

 perature. Dehydrate and imbed in celloidin. Stain in phosphotungstic acid 

 haematoxylin twenty-four hours. Wash in water, dehydrate, clear in oleum 

 origani cretici, and mount in xylol-balsam. 



Picric acid and acid fuchsin (Van Gieson's stain). 



For connective tissue : 



1 per cent, aqueous solution acid fuchsin, 5 cc. 



Saturated aqueous solution picric acid, 100 cc. 



The stain is best for the coarse fibrillae. It is sometimes better to increase the 

 proportion of acid fuchsin. Fix the tissue in chrome salts or in corrosive subli- 

 mate ; stain deeply in alum haematoxylin ; wash in water ; stain from three to 

 live minutes in the picric acid fuchsin solution ; dehydrate in 95 per cent, 

 alcohol ; clear in oleum origani cretici ; mount. 



For the nervous system : 



1 per cent, aqueous solution acid fuchsin, 15 cc. 



Saturated solution picric acid, ... 50 cc. 



Distilled water, 50 cc. 



Stain as for connective tissue. Nuclei are blueish red ; ganglion cells and pro- 

 cesses, red ; axis-cylinders, brownish red ; myelin sheaths, yellow ; neuroglia 

 fibers, red. 



Professor George W. Card, Geological Survey, New South Wales, writes 

 from Sidney, as follows : 



" It may interest you to know that additional evidence of the existence of 

 elevated foraminiferal oozes in the South Pacific has come to hand from New 

 Caledonia. Specimens of a limestone from Magenta, near Noumea, collected by 

 F. D. Power, F. G. S., are of this nature. One specimen consists largely of a 

 diversity of calcareous organisms, among which Nummuloid, Foraminifera, and 

 Globigerina were conspicuous. In addition to numerous rounded fragments of 

 other rocks, angular grains of quartz are present. Another specimen was an 

 indurated semi-crystallized cream-colored limestone, breaking with an even 

 fracture. Under the microscope this is seen to be a genuine Globigerina ooze 

 consisting entirely of the remains of Globigerina and allied forms." 



Professor Card enclosed an abstract of the meeting of the Linnean Society of 

 New South Wales, held at Linnean Hall, Sidney, New South Wales, at which 

 the following papers were read : 



Contribution to Australian Ichthyology, by J. Douglass Ogilby, Notes from 

 the Botanic Gardens, Sidney, by J. H. Maiden and D. Betche, Description of 

 a new Ophuiran, by H. Farquar. Dr. F. Tidswell gave a summary of what is 

 known as " tick fever " in cattle, and in illustration exhibited a comprehensive 

 series of preserved specimens, micrographs, and microscopical preparations of 

 ticks, and of the tick fever, haematozoon Pyrosoma bigeminum. 



