Journal of Applied Microscopy. 605 



larger suspensor cell facing the neck of the archegonium, and a smaller embry- 

 onal cell which divides into eight cells, the lower four producing the foot, and 

 the other four the shoot and first root. Lateral roots originate from the cortex 

 and not from the pericambium, as stated by Van Tieghem. 



In discussing relationships the writer comes to the conclusion that Lycopodium 

 should not be placed near Selaginella. The gametophyte bears some resem- 

 blance to that of the mosses, but the sporophyte is nearer that of the higher 

 plants. The suggestion that Lycopodium should be broken up into several 

 genera will probably meet with considerable opposition. 



c. J. c. 



^ ., „ . ^, ^ , . , It has long been known that in Homer- 



Fullmer, E. L. The Development of the ° 



Microsporangia and Microspores in Hemero- ocallis fulva the mother-cell often pro- 



callisfulva. Bot. Gaz., 28 : 81-88, pi. 7-8, j^^^gg j-j^qj-c than the usual four 



microspores. When first described 

 the origin of the extra nuclei was attributed to a mitosis of one or more of the 

 nuclei of the tetrad. More recently their origin has been attributed by Juel 

 {Cytologische StndieTi) to chromosomes, which in some way have become isolated 

 during one of the divisions of the spore-mother-cell. The present writer returns 

 to the earlier view. 



The tube nucleus frequently divides by direct division, sometimes giving 

 rise to six or eight nuclei. This is the fourth plant in which such a division has 

 been described, the previous cases being Lilium tigrmum, L. aiiratum, and 

 Eichhornia crassipes. C. J, C. 



^. , „, ^. , , In introducing the subject the com- 



Van Tieghem, Ph. Structure de quelques ° . 



ovules et parti qu'on en peut tirer pour plaint is made that in systematic works 

 ameliorer la classification. Jour, de Bot., qvuIcs are superficially described and 

 12: 197-220, 1898. ^ ^ 



inaccurately figured. The results 



recorded here are based upon an examination of the ovules of twenty-six families 

 of the Seminatae, one of the two groups into which he divides the Dicotyls. The 

 Seminatae he divides into two classes, the Crassinucellatae, or those in which the 

 nucellus is rather thick and retains its form until the time of fertilization, and 

 the Tenuinucellatae, or those in which the nucellus consists of a single layer of 

 cells. Each of these classes is subdivided into two orders, the Unitegminatae 

 and the Bitegminatae, according as they have one or two integuments. 



The investigations have brought us a large amount of information in regard 

 to the ovule, but it is doubtful whether a classification based so largely upon 

 one character will meet with any general acceptance. 



c. J. c. 



Guignard, L. Les centres cinetiques chez les Various fixing agents were employed, 

 vegetaux. Ann. des Sci. Nat. Bot., Ser. but the best results were obtained with 



VIII, 5: 178-220, pi. 9-1 1, 1898. T^, • . ri ■ 1 • ^u f 11 • 



^ ^ Hemmmgs fluid m the following pro- 



portions: water 100 cc, chromic acid one-half g., osmic acid one-half g., and 

 acetic acid two cc. The most satisfactory staining was secured by using a 

 mixture of methyl green, acid fuchsin, and orange G in aqueous solution. The 

 proportions should be such that, after about twelve hours, the chromosomes of 



