Journal of Applied Microscopy. 615 



extraneous organisms, among which the pyogenic bacteria predominated. It 

 appears that certain insects perpetuate or actually spread diseases, and that they 

 may be potent agents in the transportation of bacteria for twenty-four or forty- 

 eight hours, or perhaps longer, after infection. E. M. Brace. 



Graham, J. S. The Preservation of Culture As a method for effectively sealing 

 Media. Ga. Jour. Med, Surg., March, 1899. ^^^^^ ^f ^^j^ure media, the writer 



recommends depressing the cotton plug and dipping the mouth of the tube into 

 boiling white beeswax after sterilization. After cooling slightly, the tubes are 

 again dipped in wax that has been cooled until it is beginning to solidify. 



E. M. Brace. 



Staining Method for Blood Preparations. This is a method for bringing OUt the 

 Deutsch. Med. Woch., August 19, 1899. ^^^j^j^ ^he neutrophilous, eosinophilous, 



and basophilous granulations, and the blood plates. The preparation is fixed 

 by the Ehrlich method, or by treating with alcohol from one-half to twenty-four 

 hours. Solution A consists of twenty parts of methylen blue with twenty parts 

 of absolute alcohol. Solution B consists of twelve parts of eosin with 28.9 parts 

 of acetone. Pour together one cc. of each of these and dip the preparation into 

 the mixture. The preparation turns blue> then red, and should be removed from 

 the stain the moment the red reaction appears. The time required varies from 

 one-half minute to ten minutes. E. M. Brace. 



NORMAL AND PATHOLOGICAL HISTOLOGY. 



Richard M. Pearce, M. D. 



Harvard Medical School, Boston, Mass., to whom all books and papers 

 on these subjects should be sent for review. 



Labbe. Le ganglion lymphatique dans las The lymph nodes from cases of ery- 

 infections aigues. La Presse Medicale. gipeias, typhoid fever, diphtheria, lobar 

 4 plates, March 22, 1899. r ' y r ' r- 



pneumonia, and broncho-pneumonia 



were studied. In erysipelas, in the adult, the lymph nodes near the inflamma- 

 tion were found to be congested. The sinuses contained desquamated cells, often 

 showing evidence of phagocytosis. The follicles were little altered. In the 

 new-born, however, the lymph node was hsemorrhagic, oedematous, the follicles 

 necrotic, and the lymph sinuses obliterated by phagocytic cells and altered lympho- 

 cytes and polymorphonuclear leucocytes. In the walls of blood vessels inflam- 

 matory changes were seen. In the adult the streptococci were seen only in the lymph 

 sinuses ; in the new-born they were seen scattered diffusely through all parts of 

 the node. 



In typhoid fever the mesenteric lymph nodes only were examined. 

 During the first week of the disease hyperaemia was the only change noticed. 

 At the height of the disease, the follicles contained lymphocytes with altered, 

 shrunken nuclei. Often the centers of follicles were necrotic. The lymph 

 sinuses were dilated and contained many large pale cells with vesicular nuclei. 

 These cells were markedly phagocytic. Some of the lymph nodes nearest the 



