Journal of Applied Microscopy. 



641 



infiltration of paraffin or celloidin. The puncture causes only a slight damage, 

 and need not reach the really valuable portion which is to furnish the median 

 longitudinal sections. 



}OVRf\fpfiiC. 



Fig. lo. Bryum. >( 200. 

 Portion of a nearly mature capsule showing operculum, 

 annulus, peristome, and three cells of the sporo- 

 genous tissue. Fixed in Flemming's weaker solu- 

 tion, stained in safranin and Delafield's hema- 

 toxylin. Fifteen microns. 



JoyR flpp M'c, 



Fig. II. .Sphagnum. X 24. 

 Longitudinal section of mature sporophyte 

 showing also the upper portion of the 

 pseudopodium and the calyptra. Chromo- 

 acetic acid, D,;lafield's hematoxylin. 

 Paraffin. Ten microns. 



Protonema and teased mounts of antheridia and archegonia may be made 

 directly in 50 per cent, glycerine without fixing or staining. They will keep 

 the green color for a long time. 



(To be continued.) 



Glycerine Jelly. — This medium, available for such a variety of pur- 

 poses, may be easily made in the laboratory. To insure the greatest possi- 

 ble transparency, the gelatin should be thoroughly washed and it should not be 

 subjected to high temperatures. Use any good photographic gelatin, place it in 

 apparatus commonly used for washing laboratory specimens, and leave it in 

 gently-running water over night. By the time it is perfectly clean, the gelatin 

 will have absorbed as much water as possible, and it may then be melted by gentle 

 heat in an equal quantity of pure glycerine. After this it should be filtered at 

 least three times. This is done by heating the funnel in hot water, after which 

 the filter paper is placed in position and hot water poured through it before 

 filtering the jelly. A crystal of thymol will keep it from molding. 



Glycerine jelly made in this way is clear and may be used for mounting fofo 

 specimens in museum jars, as well as for mounting microscopical objects, etc. 



