Journal of Applied Microscopy. 645 



ANIMAL BIOLOGY. 



Agnes M. Claypole. 



Separates of papers and books on animal biology should be sent for review to 



Agnes M. Claypole, Sage College, 



Ithaca, N. Y. 



CURRENT LITERATURE. 



Dimmer, F. Eine Modification der Celloidin- The author used the method described 

 serienmethode. Zeitschr. fur wiss. mikros., for celloiden material Stained in toto or 

 • 44-4 . I 99- £q^ preparations to be stained by the 



Weigert or Weigert-Pal process. For the latter cases the author attempted to 

 find a process by which the coating of the celloidin sections with two layers of 

 collodion could be avoided, in this way leaving one side free for the penetration 

 of stains. Obregia poured a sugar solution over a glass plate on which the sec- 

 tions could be placed. This plate so prepared was treated with a solution of 

 photoxylin, which dissolved off, the sugar and the sections contained in the coat- 

 ing of photoxylin can be lifted from the glass together. Instead of the sugar, 

 the author used a gelatin solution (gel. 16 g., warm water 300 g.). Large 

 glass plates previously warmed were coated thinly, and laid horizontally, and 

 protected from dust till dry, which was usually in about two days. The sec- 

 tions were transferred in the usual way with tissue paper to the glass prepared 

 in this way, and washed over with seventy per cent, alcohol ; this is absorbed 

 with paper and the sections well pressed down to the glass at the same time. A 

 piece of silk paper for naming the sections can be fastened on at the same time 

 in the same way. Photoxylin solution (photox. 6 g. absol. alcoh. and ether - 

 100 cc), is poured over all and allowed to dry of¥ somewhat ; the plate is then 

 placed in a vessel with water of from 50° to 55° C, after scraping 

 the photoxylin layer from the edge of the glass with a sharp knife to allow the 

 water to penetrate and dissolve out the gelatin. The sections fastened together 

 with photoxylin are readily separated from the glass, and can be transferred to 

 stains by means of tissue paper, and eventually carried through clearer. If the 

 photoxylin is not easily separated from the glass, longer treatment with warm 

 water will loosen it. 



The process is easy and simple ; the gelatin wash to the glass is quickly 

 done and dried, and the sections if well pressed down are not disturbed by the 

 pouring on of photoxylin. This process, as already mentioned, is only for toto 

 stained preparations or those to be treated by the Weigert method. In other 

 stains the small amount of gelatin remaining colors so as to spoil the preparation. 



A. M. c. 



Rosin, H. TJeber ein neue Gruppe von Ani- Rosin States that an entirely new stain- 



linfarbstoffen, ihre Bedeutung fiir die Bio- . , • i ■ ^ ^ ■ • i 



chemie der Zelle und ihre Verwendbarkeit l"g material IS made by mixmg solu- 



fur die Gewebsfarbung. Berliner klin. tions of acid eosin and basic methylin- 



Wochenschr., pp. 251, 1898. ,, i.- 1. 1 n ,, -j • .1 



^^ -^ ^ blue, which he calls " acid-eosm-methy- 



lin-blue." In a similar way he obtains a new series of crystallized stains by the 



union of other acid and basic anilin colors. To these belong the substance 



