[Proc. Eoy. Soc. Victoria, 24 (N.S.), Pt. II., 1911.] 



Art, XXIV. — Protozoa Parasitic in the Large Intestine 



of Australian Frogs. 



Part IT. 



By JANET W. RAFF, M.Sc. 



(Government Research Bursar, Melbourne University.) 



(With Plates LXX. and LXXI.). 

 [Head 14th December, 1911.]. 



In continuing my investigations into the Protozoa living in the 

 large intestine of Australian frogs, I have obtained for examina^ 

 tion, besides additional specimens of those frogs mentioned in 

 Part I., the following: — Hyla lesueurii (several specimens); 

 Helioporus pictus, Pseudophryne bibronii, Crinia signifera and 

 Crinia froggatti (two specimens of each) ; Pseudophryne semi- 

 mar morata and Hyperolia inarmorata (one specimen of each). 



In these last seven species I have found nothing new with the 

 exception of a new Opalina in C. signifera and a new Amoeba 

 in Hyla lesueurii. I have also to describe two more new spe<;ies 

 of Opalina from Limnodynastes dorsalis, together with some 

 abnormal forms from a frog of the same species. Most of the 

 frogs contain the usual Trichomonads, Nyctotherus cordiformis, 

 and one or more species of Opalina. The new Amoeba from 

 Hyla lesueurii also occurred in H. aureu and H. peronii, as well 

 as in tadpoles from various localities. 



Opalina tenuis, n. sp. 



This binucleated form was found in the large intestine of two 

 specimens of the brown froglet, Crinia signifera, one coming 

 from Narbethong, near Healesville, the other from Mentone, for 

 both of which my thanks are due to Miss 0. B. Davies. B.Sc. I 

 have also found it in another small frog from Narbethong, which 

 I take to be Hyperolia marmorata. They were present in 



