Feb. IS, 1915 



Organic Phosphoric Acid of Rice 



429 



The analyses of the barium phytate are given in Table III and are 

 calculated to the water-free basis. The barium and phosphorus content 

 is lower than that reported by Anderson for tribarium-inosite-hexaphos- 

 phate. Whether the barium phytate obtained was composed of a single 

 salt of inosite is not absolutely certain. 



Table III. — Analyses of barium phytate calculated to the water-free basis 



UNPOLISHED RICE « 



J 0.5107 gm. lost 0.0039 gm. H:0=o.76 per cent of moisture. 

 ^ 0.9717 gm. lost 0.0139 gm. H20=i.33 per cent of moisture. 



Inosite was prepared from the barium phytate of rice bran by heating 

 in sealed tubes to 150° C. about 2 gm. of the salt with 20 c. c. of 30 per 

 cent sulphuric acid for five hours. 



The sulphuric acid was precipitated with barium hydroxid, the excess 

 of barium removed by carbon dioxid, and the filtrate evaporated to 

 dryness. The residue was extracted with hot water and filtered. The 

 inosite was precipitated by ether and alcohol and rccrystallized three 

 times as minute needles. These gave the Scherer reaction and melted 

 at 223° C, uncorrected. 



Thanks are due Dr. W. P. Kelley, of the Hawaii Experiment Station, 

 who suggested this work on phylin in rice and gave helpful advice 

 throughout. 



