Mar. 25, 1915 



Dissemination of Chestnut-Blight Fungus 



517 



nearest lesion was on a branch 13 feet to the north. The standard sugar- 

 tube method of making a quantitative bacteriological analysis of air was 

 employed. The bottle was refilled with 4 liters of water at intervals 

 of 20 or 30 minutes, thus making the aspiration practically continuous. 

 One sugar tube was generally used each day, and the quantity of air 

 drawn through each tube averaged 58 liters, with a maximum of 96 liters. 

 The medium employed was a 3 per cent dextrose agar, with a reaction 

 of +10. Ten plates were poured for each test and were incubated 

 and the colonies counted in the same way as those in the experiments 

 with the water spore traps (p. 520). 



Table XIV. — Summary of results of aspirator tests in IQI3 at West Chester, Pa. 



Sugar 

 tube 

 No. 



2 



3 

 4 

 5 

 6 



7 

 8 



9 

 10 

 II 

 12 

 13 

 14 

 15 

 16 



17 

 18 



19 

 20 

 21 

 22 

 23 

 24 

 25 

 26 



27 

 28 



29 

 30 

 31 

 32 

 33 

 34 

 35 

 36 

 37 

 38 

 39 

 40 



41 



Date of aspiration. 



Aug. 19 



Aug. 20 



Aug. 21 



Aug. 22 



Aug. 23 



Aug. 24 



Aug. 25 



Aug. 26 



Aug. 27 



....do 



Aug. 28 



Aug. 29 



Aug. 30 



Aug. 31 



Sept. I 



Sept. 2 



Sept. 3 



Sept. 4 



Sept. s 



....do 



Sept. 6 



Sept. 7 



Sept. 7 and 8. 



vSept. 8 



Sept. 9 



Sept. 10 



Sept. II 



Sept. 12 



Sept. 13 



Sept. 14 



Sept. 15 



vSept. 16 



Sept. 17 



Sept. 18 



do 



Sept. 19 



Sept. 20 



Sept. 21 



do 



Sept. 22. .... . 



Sept. 23 



Rainfall." 



Inches. 

 O. 40 

 O 

 O 

 O 

 •25 



175 



37 



■095 



.26 



Average. 



.68 

 .09 



•43 

 . 10 



•73 



Quantity 

 of air 

 repre- 

 sented. 



Number of 



bacteria 



and 



yeasts 



per liter. 



Liters. 

 12 

 28 



36 

 48 

 68 

 56 

 56 

 56 

 47 

 12 

 68 

 40 

 60 

 56 

 92 

 72 

 72 

 56 

 68 

 4 

 76 

 64 

 12 

 76 

 60 

 76 

 72 

 80 

 68 

 72 

 72 

 72 

 80 



52 

 64 

 96 

 76 

 40 

 28 

 ^2 

 80 



26. 42 

 3-21 



•83 

 2. 29 

 1.47 

 2.86 

 3-96 

 4.64 

 2. 12 

 3^75 

 1-35 



2-5 

 I. 2S 

 I. 16 



1-25 

 I. 18 

 1.32 

 1.78 

 I. 69 

 16. 78 



1. 12 

 4. 61 

 6.23 



•39 

 .16 



2. 17 



•59 



■ 14 



.62 



.41 

 1. 00 

 7^59 



• 15 

 1.04 



•59 

 1.50 

 1.32 

 2.30 



•87s 



Total 

 number 

 of fungi 

 per liter. 



Number of 

 spores of 

 Endothia 

 parasitica 

 per liter. 



57.9 2.91 



4. 16 

 4.28 

 3-05 

 5- 21 

 17-65 

 II. 07 

 3-96 

 8-93 

 8-3 

 5-0 

 4.04 



5-875 



3^9i 



7.67 



38. 37 

 1.87 

 7.91 

 36. 60 

 20. 00 

 6.2s 

 8.09 

 3-90 

 8-33 

 4.86 

 I. 41 

 6. 90 

 1.94 

 2.58 

 1.91 

 1. II 

 I. 18 



'•25 



I. 12 

 14-23 



5-94 

 4.27 



'•3i 

 4- 50 

 6.25 

 1.82 

 I- 31 



Number of 

 species 

 of fungi. 



•35 



•42 



.080 



•03 



4 

 9 

 9 



7 

 3 

 5 

 7 



10 

 II 



9 

 12 



9 



7 



10 

 II 



8 

 12 

 ir 

 II 



7 



12 



13 

 10 



lO 



5 

 10 



7 



9 



6 



)0 



6 

 8 

 8 



14 



8.8 



« Al! rains occurred durinc the night previous to the date of aspiration, except on Aue. a? 



