H. T. P. IJakkkk AM) (). (iuovK 97 



the plate originally coiitaiiiocl only 8-10 colonies. A plate culture in 

 the liquid state has a pronounced smell of ammonia 



In bouillon agar at IS'' C. stab cultures give feeble growth in three 

 days, spreading out on the surface. Streak cultures form in three days 

 a fiat, glistening, smooth-edged, whitish, spreading growth. In plate 

 cultures the colonies are visible after 48 hours. The surface colonies 

 after three days are small, round, raised, glistening and whitish, later 

 spreading out over the surface. Submerged colonies are white and remain 

 very small. 



On potato a raised, yellowish-white, broad, smooth-edged growth 

 is formed after eight days at 18° (J. On parsnip and carrot a feeble 

 growth is observed after five days. On turnip no development takes 

 place. 



In sterilised milk a good growth is obtained. No curdling takes 

 place in eight days at 18° C, but the milk is eventually very slowly 

 peptonised. 



No fermentation takes place in 2 % solutions of saccharose, maltose, 

 glucose, laevulose, or lactose, to which 1 % peptone was also added. 



Old cultures in glucose-peptone solution exhibit a pronounced 

 greenish fluorescence. This has not been observed in the case of any 

 other media. 



There is no indol reaction given in eight-day old cultures. 



From these characters it appears that the organism is a species of 

 Pseudomonas. So far it has not been identified with any hitherto 

 described form ; but on account of its wide distribution and occurrence 

 in the soil it is possible that it may be known to soil bacteriologists as 

 one of the ammonia-forming types. 



Ann. Biol, i 



